Reviewed, UniProtKB/Swiss-Prot Q65MS6 (PUR5_BACLD)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	BLi00701, BL01484

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_0000258331
Experimental info
	Sequence conflict	1	1	M → ML in AAU39638. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q65MS6-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 1B68F9EBA0283609	FASTA	346	36,584
	10 20 30 40 50 60 MSESYKNAGV DIEAGYEAVK RMKKHVERTK RIGAMGGLGG FGGMFDLSEL PYKQPVLISG 70 80 90 100 110 120 TDGVGTKLKL AFAMDKHDTI GIDAVAMCVN DVLAQGAEPL FFLDYLAVGK ADPVKIEEIV 130 140 150 160 170 180 KGVAEGCVQS GSALVGGETA EMPGLYTEDE YDIAGFSVGA AEKDGIVTGE NISEGHLLIG 190 200 210 220 230 240 LSSSGLHSNG FSLVRKVLLE DAGLNLDETY APFERPLGEE LLEPTKIYVK PVLEAVKSGK 250 260 270 280 290 300 IAGMAHVTGG GFIENLPRMM PDGLGVEIDI GSWPVPPIFP FIQEKGGLKS EEMFNVFNMG 310 320 330 340 IGFVLAVKEE DMTDVIQTLE NNGEKAYLIG RVKAGSGVVF GGAGLS

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU39638.1. CP000002 Genomic DNA. Translation: AAU22287.1.
RefSeq	YP_077925.1. YP_090331.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65MS6.
Genome annotation databases
GeneID	3031170. 3099228.
GenomeReviews	Gene locus BLi00701 in contig AE017333_GR. Gene locus BL01484 in contig CP000002_GR.
KEGG	bld:BLi00701. bli:BL01484.
NMPDR	fig|279010.5.peg.1009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65MS6.
OMA	VHGLAHI.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01484-MON.
Family and domain databases
HAMAP	MF_00741. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BACLD

Accession

Primary (citable) accession number: Q65MS6
Secondary accession number(s): Q62Y71

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	November 3, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)