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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791Charge relay systemUniRule annotation
Active sitei154 – 1541Charge relay systemUniRule annotation
Active sitei178 – 1781Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-724-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:BLi00731, BL00601
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000608 Componenti: Chromosome UP000000606 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000241071Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BL00601.

Structurei

3D structure databases

ProteinModelPortaliQ65MP8.
SMRiQ65MP8. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65MP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLFDHKISE LKRLIHNKEI SISDLVDESY KRIHEVDGKV QAFLQLDEEK
60 70 80 90 100
ARAYAKELDE ALDTRDEHGL LFGMPIGIKD NIVTKDLRTT CASKILENFD
110 120 130 140 150
PIYDATVVER LHEAEAVTIG KLNMDEFAMG SSTENSGFKK TKNPWNLETV
160 170 180 190 200
PGGSSGGSAA AVAAGEVPFS LGSDTGGSIR QPASFCGVVG LKPTYGRVSR
210 220 230 240 250
YGLVAFASSL DQIGPITRSV EDNAYLLQAI SGVDKMDSTS ANVDVPDYLS
260 270 280 290 300
ALTGDIKGLK IAVPKEYLGE GVSEEAKQSV LEALKVLESL GATWEEVSLP
310 320 330 340 350
HSKYALATYY LLSSSEASAN LARFDGIRYG YRTDNADNLI DLYKQTRSEG
360 370 380 390 400
FGNEVKRRIM LGTFALSSGY YDAYYKKAQK VRTLIKKDFE DVFANYDVII
410 420 430 440 450
GPTTPTPAFK IGEKTSDPLT MYANDILTIP VNLAGVPGIS VPCGFANGLP
460 470 480
LGLQIIGKHF DESTVYRVAH AFEQATDHHK AKPEL
Length:485
Mass (Da):52,882
Last modified:October 25, 2004 - v1
Checksum:i4D3F944611FFDEE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU39666.1.
CP000002 Genomic DNA. Translation: AAU22316.1.
RefSeqiYP_006712136.1. NC_006322.1.
YP_077954.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU22316; AAU22316; BL00601.
AAU39666; AAU39666; BLi00731.
KEGGibld:BLi00731.
bli:BL00601.
PATRICi18946993. VBIBacLic203714_0721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU39666.1.
CP000002 Genomic DNA. Translation: AAU22316.1.
RefSeqiYP_006712136.1. NC_006322.1.
YP_077954.1. NC_006270.3.

3D structure databases

ProteinModelPortaliQ65MP8.
SMRiQ65MP8. Positions 1-485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BL00601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU22316; AAU22316; BL00601.
AAU39666; AAU39666; BLi00731.
KEGGibld:BLi00731.
bli:BL00601.
PATRICi18946993. VBIBacLic203714_0721.

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-724-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.

Entry informationi

Entry nameiGATA_BACLD
AccessioniPrimary (citable) accession number: Q65MP8
Secondary accession number(s): Q62Y42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: October 25, 2004
Last modified: April 1, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.