Q65M66 (AROD_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	BLi00939, BL00001

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325519
Sites
	Active site	144	1	Proton acceptor By similarity
	Active site	171	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65M66 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 80C9F8560A258A87	FASTA	254	27,695
	10 20 30 40 50 60 MKKLVKVRGI TLGEGRPKIC VPLVGKNLAQ LKEEAAYFQT LDADIAEWRA DFYEDVDDVD 70 80 90 100 110 120 QVCAALAEIR GLLKDTPLIF TFRSAAEGGE KEISTARYFS LNEAVVKTGL ADVIDIELFN 130 140 150 160 170 180 DEEQVKALVE TAHGHEVSVI ISNHDFEKTP AAEEIVSRLR KAQELGADLP KIAVMPETTA 190 200 210 220 230 240 DVLRLLEATC TMNEKYADRP IITMSMAGKG IISRLAGEVF GSAMTFGAAK KASAPGQIAA 250 GELKEILHIL HRNL

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU22502.1. AE017333 Genomic DNA. Translation: AAU39848.1.
RefSeq	YP_078140.1. NC_006270.3. YP_090541.1. NC_006322.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQN based on UniProtKB P24670.
ProteinModelPortal	Q65M66.
SMR	Q65M66. Positions 3-252.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65M66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000054520; EBBACP00000053051; EBBACG00000054511. EBBACT00000058723; EBBACP00000057156; EBBACG00000058714.
GeneID	3029790. 3101155.
GenomeReviews	Gene locus BLi00939 in contig AE017333_GR. Gene locus BL00001 in contig CP000002_GR.
KEGG	bld:BLi00939. bli:BL00001.
NMPDR	fig|279010.5.peg.1524.
PATRIC	18947413. VBIBacLic203714_0912.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0710.
GeneTree	EBGT00050000001972.
HOGENOM	HBG732926.
OMA	IAKIAVM.
ProtClustDB	PRK02412.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI00939-MONOMER. BLIC279010:BL00001-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_BACLD

Accession

Primary (citable) accession number: Q65M66
Secondary accession number(s): Q62XK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program