Q65M11 (GLPK_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BLi00995, BL02903

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Glycerol kinase HAMAP MF_00186		PRO_1000020702
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65M11 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 5E0F21418E1FF8B0	FASTA	496	55,183
	10 20 30 40 50 60 MEKYILSLDQ GTTSTRAIVF NKAGEIVHIA QKEFRQYFPN PGWVEHNANE IWGSVLSVIA 70 80 90 100 110 120 SALSESGIEA GQIAGIGITN QRETTVVWDK HTGKPVYNAI VWQSRQSAEI CQELKEKGYE 130 140 150 160 170 180 ETIREKTGLL IDPYFSGTKV KWILDHVEGA REKAENGDLL FGTIDSWLIW KMSGGKAHVT 190 200 210 220 230 240 DYSNASRTLM FNIYDLKWDD ELLDILGVPK SMLPEVKPSS HVYAETVDYH FFGKNIPIAG 250 260 270 280 290 300 AAGDQQAALF GQACFEEGMV KNTYGTGCFM LMNTGEKAIK SEHGLLTTIA WGIDGKVEYA 310 320 330 340 350 360 LEGSVFVAGS AIQWLRDGLR MFKDAKESEK YAVRAESADG VYVVPAFVGL GTPYWDSDVR 370 380 390 400 410 420 GAVFGLTRGT TKEHFIRATL EALAYQTKDV LDAMKEDSGI PVKTLRVDGG AVKNNFLMDF 430 440 450 460 470 480 QGDILDVPVE RPEINETTAL GSAYLAGLAV GFWSDRSEIK DQWQLDKRFE PKMEEKERES 490 LYNGWKKAVN AARAFK

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU22560.1. AE017333 Genomic DNA. Translation: AAU39903.1.
RefSeq	YP_078198.1. NC_006270.3. YP_090596.1. NC_006322.1.
3D structure databases
HSSP	HSSP built from PDB template 1XUP based on UniProtKB O34153.
ProteinModelPortal	Q65M11.
SMR	Q65M11. Positions 4-489.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65M11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000056110; EBBACP00000054641; EBBACG00000056101. EBBACT00000061992; EBBACP00000060425; EBBACG00000061983.
GeneID	3029876. 3098715.
GenomeReviews	Gene locus BLi00995 in contig AE017333_GR. Gene locus BL02903 in contig CP000002_GR.
KEGG	bld:BLi00995. bli:BL02903.
NMPDR	fig|279010.5.peg.1669.
PATRIC	18947533. VBIBacLic203714_0971.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
GeneTree	EBGT00050000001374.
HOGENOM	HBG511469.
OMA	ALYGQLC.
PhylomeDB	Q65M11.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI00995-MONOMER. BLIC279010:BL02903-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BACLD

Accession

Primary (citable) accession number: Q65M11
Secondary accession number(s): Q62XE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program