Q65LF6 (GUAC_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	BLi01200, BL05105

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	GMP reductase HAMAP MF_01511		PRO_0000093751
Regions
	Nucleotide binding	204 – 227	24	NADP Potential
Sites
	Active site	175	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65LF6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 9767F95E143A670E	FASTA	326	35,893
	10 20 30 40 50 60 MENVFDYEDI QLIPAKCIVK SRSECDTSVQ FGGRTFKLPV VPANMQTIID EKLAVSLAEN 70 80 90 100 110 120 GYFYVMHRFE PETRIDFIKD MKARGLFSSI SVGVKDEEYA FIEELTRENL TPEYITIDIA 130 140 150 160 170 180 HGHSNAVINM IQHIKKHLPD SFVIAGNVGT PEAVRELENA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCAKAA SKPIIADGGI RTHGDIAKSV RFGATMVMIG SLFAGHEESP 250 260 270 280 290 300 GATIEKDGKL YKEYFGSASE YQKGEKKNVE GKKMYVEHKG AIMDTLTEME QDLQSSISYA 310 320 GGNKLDAIRN VDYVIVKNSI FNGDQY

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU40108.1. CP000002 Genomic DNA. Translation: AAU22762.1.
RefSeq	YP_078400.1. NC_006270.3. YP_090801.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65LF6.
SMR	Q65LF6. Positions 1-321.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65LF6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000054478; EBBACP00000053009; EBBACG00000054469. EBBACT00000060372; EBBACP00000058805; EBBACG00000060363.
GeneID	3030188. 3099019.
GenomeReviews	Gene locus BLi01200 in contig AE017333_GR. Gene locus BL05105 in contig CP000002_GR.
KEGG	bld:BLi01200. bli:BL05105.
NMPDR	fig|279010.5.peg.2281.
PATRIC	18947967. VBIBacLic203714_1188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0516.
GeneTree	EBGT00050000000995.
HOGENOM	HBG298985.
OMA	PDYITID.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI01200-MONOMER. BLIC279010:BL05105-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BACLD

Accession

Primary (citable) accession number: Q65LF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program