Q65LE8 (ARGB_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BLi01208, BL03243

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112584
Regions
	Region	41 – 42	2	Substrate binding By similarity
Sites
	Binding site	63	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	215	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65LE8 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 9CB3178CF22CAC39	FASTA	258	27,777
	10 20 30 40 50 60 MGKTIVFKCG GSVIRELSDE FFQNLKDLTE SGWKIALVHG GGPDITKMLK KLNIRTEFVN 70 80 90 100 110 120 GQRKTTKAVL EVAEMVLSGS INKFFVAELT KHGLPAVGVS GKDGGLLLAD YLNEKEYGQV 130 140 150 160 170 180 GRIKKVNGQM AEALMEKGFI PVIAPLSMTD GCETLNVNAD LAASAVAAAL RADKLMFVTD 190 200 210 220 230 240 VKGIMKNEEM LKELTPEDIE SLIAEGVISG GMIPKVHSAK EALTDEVEEV MIVSGKGSFL 250 TKDDFIGTKI IKQKEAVS

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU40116.1. CP000002 Genomic DNA. Translation: AAU22770.1.
RefSeq	YP_078408.1. NC_006270.3. YP_090809.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65LE8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65LE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000054630; EBBACP00000053161; EBBACG00000054621. EBBACT00000060957; EBBACP00000059390; EBBACG00000060948.
GeneID	3030197. 3101134.
GenomeReviews	Gene locus BLi01208 in contig AE017333_GR. Gene locus BL03243 in contig CP000002_GR.
KEGG	bld:BLi01208. bli:BL03243.
NMPDR	fig|279010.5.peg.2377.
PATRIC	18947985. VBIBacLic203714_1197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
GeneTree	EBGT00050000001971.
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	Q65LE8.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI01208-MONOMER. BLIC279010:BL03243-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BACLD

Accession

Primary (citable) accession number: Q65LE8
Secondary accession number(s): Q62WT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program