Q65LA0 (PRPE_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BLi01257, BL05112

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297700

Sequences

	Sequence		Length	Mass (Da)
	Q65LA0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 004D8C97109538C7	FASTA	246	27,936
	10 20 30 40 50 60 MKYDVIGDIH GCYDEMIALI EKLGYSLTSG VPVHPENRTL VFLGDLTDRG PKSLEVVEFV 70 80 90 100 110 120 GRAVEDGAVR YVPGNHCNKL YRFFKGNPVK LQHGLETTAA EYRNLPKQKQ KDIRDLFMRL 130 140 150 160 170 180 YEKAPLYEIL HNGELVVAHA GIKAEDIGKK PVGKVKQFVL YGDITGETLP DGRPVRRDWA 190 200 210 220 230 240 AHYRGEPWVV YGHTPVKNPR MIHHTINIDT GCVFGNRLTA FRFPELTTES VPSSLPYDES RFRMEP

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU22819.1. AE017333 Genomic DNA. Translation: AAU40164.1.
RefSeq	YP_078457.1. NC_006270.3. YP_090857.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65LA0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65LA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000054649; EBBACP00000053180; EBBACG00000054640. EBBACT00000060298; EBBACP00000058731; EBBACG00000060289.
GeneID	3030263. 3100058.
GenomeReviews	Gene locus BLi01257 in contig AE017333_GR. Gene locus BL05112 in contig CP000002_GR.
KEGG	bld:BLi01257. bli:BL05112.
NMPDR	fig|279010.5.peg.2474.
PATRIC	18948087. VBIBacLic203714_1248.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0639.
GeneTree	EBGT00050000002430.
HOGENOM	HBG724457.
OMA	YVPGNHC.
PhylomeDB	Q65LA0.
ProtClustDB	PRK13625.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI01257-MONOMER. BLIC279010:BL05112-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
KO	K01525.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
ProtoNet	Search...

Entry information

Entry name

PRPE_BACLD

Accession

Primary (citable) accession number: Q65LA0
Secondary accession number(s): Q62WN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program