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Q65KX8 (BGAL1_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase YesZ

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Probable rhamnogalacturonan beta-galactosidase
Gene names
Name:yesZ
Ordered Locus Names:BLi01382, BL03780
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length663 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May play a role in the degradation of rhamnogalacturonan derived from plant cell walls By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subunit structure

Homotrimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 663663Beta-galactosidase YesZ
PRO_0000367025

Regions

Region346 – 3494Substrate binding By similarity

Sites

Active site1461Proton donor By similarity
Active site2971Nucleophile By similarity
Metal binding1111Zinc By similarity
Metal binding1541Zinc By similarity
Metal binding1561Zinc By similarity
Metal binding1591Zinc By similarity
Binding site1071Substrate By similarity
Binding site1451Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65KX8 [UniParc].

Last modified March 24, 2009. Version 2.
Checksum: C2044F388107C6C6

FASTA66374,214
        10         20         30         40         50         60 
MNGKLYHGAC FYPELWDEDV LDEDIRMMER IGINVVRIGE FAWSRMEPEK GRIDVGFFAD 

        70         80         90        100        110        120 
VIRKLRDNKI ETVMCTPTAT PPIWLTHGHP ERMHVNEKGE TMGHGSRQHA CTNHPYFRER 

       130        140        150        160        170        180 
ARLIIKHIAK EIGELPGLIG WQLDNEFKCH VAECICETCR TLWHKWLEDR YQTIDRLNEA 

       190        200        210        220        230        240 
WGTGVWSETY QCFEQVPQPG PTPFLHNSSL RTMYQLFSMD KISEFAREQA EVIRAYSDAP 

       250        260        270        280        290        300 
ITHNSSVMFG VDHEDLFKSL DFASFDTYAS QENSQAFLFN CDLWRNIKKG RPFWIMETSP 

       310        320        330        340        350        360 
SYSASLESYA APHQNGYLKA EAVSSYALGG AAFCYWLWRQ QRAGSEQPHG SVLSAWGEPD 

       370        380        390        400        410        420 
VGYENVLEAE RARREVEHIM LATAPLQAET AVVYSDRAKV FLKTEPHRGL HYRTLITEFY 

       430        440        450        460        470        480 
DRLLKMGIHR DVILEGSPLD GYKLLFTPFI HYLPPAFIKK AEAFAQSGGI WIAGPLTGGR 

       490        500        510        520        530        540 
TEHHTIHTDC GLGPLEKCSG VKTLFTFPMD ERNSSGTAFG VKAPLSLWSA VFEAGGTKAV 

       550        560        570        580        590        600 
GMIEKGPASG KAFITEHKCG KGKIVMLGSM PAGEAGDIMM KKLISHYAEE AGVEQKTDVT 

       610        620        630        640        650        660 
PGTVVAPRKG ADGLVWVVIN MDGKGGAVTL DGNGTDLLSG RPVTGRVTLG PHDYRVILLS 


ENK 

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References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017333 Genomic DNA. Translation: AAU40286.1.
CP000002 Genomic DNA. Translation: AAU22933.1.
RefSeqYP_006712760.1. NC_006322.1.
YP_078571.1. NC_006270.3.

3D structure databases

ProteinModelPortalQ65KX8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL03780.

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU22933; AAU22933; BL03780.
AAU40286; AAU40286; BLi01382.
GeneID3030421.
3101523.
KEGGbld:BLi01382.
bli:BL03780.
PATRIC18948341. VBIBacLic203714_1374.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000095202.
KOK01190.
OrthoDBEOG6HMX9F.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-1371-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL1_BACLD
AccessionPrimary (citable) accession number: Q65KX8
Secondary accession number(s): Q62WC5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: June 11, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries