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Protein

Beta-galactosidase YesZ

Gene

yesZ

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May play a role in the degradation of rhamnogalacturonan derived from plant cell walls.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071SubstrateBy similarity
Metal bindingi111 – 1111ZincBy similarity
Binding sitei145 – 1451SubstrateBy similarity
Active sitei146 – 1461Proton donorBy similarity
Metal bindingi154 – 1541ZincBy similarity
Metal bindingi156 – 1561ZincBy similarity
Metal bindingi159 – 1591ZincBy similarity
Active sitei297 – 2971NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1371-MONOMER.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase YesZ (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Probable rhamnogalacturonan beta-galactosidase
Gene namesi
Name:yesZ
Ordered Locus Names:BLi01382, BL03780
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 663663Beta-galactosidase YesZPRO_0000367025Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi279010.BL03780.

Structurei

3D structure databases

ProteinModelPortaliQ65KX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni346 – 3494Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Curated

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000095202.
KOiK01190.
OrthoDBiEOG6HMX9F.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65KX8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGKLYHGAC FYPELWDEDV LDEDIRMMER IGINVVRIGE FAWSRMEPEK
60 70 80 90 100
GRIDVGFFAD VIRKLRDNKI ETVMCTPTAT PPIWLTHGHP ERMHVNEKGE
110 120 130 140 150
TMGHGSRQHA CTNHPYFRER ARLIIKHIAK EIGELPGLIG WQLDNEFKCH
160 170 180 190 200
VAECICETCR TLWHKWLEDR YQTIDRLNEA WGTGVWSETY QCFEQVPQPG
210 220 230 240 250
PTPFLHNSSL RTMYQLFSMD KISEFAREQA EVIRAYSDAP ITHNSSVMFG
260 270 280 290 300
VDHEDLFKSL DFASFDTYAS QENSQAFLFN CDLWRNIKKG RPFWIMETSP
310 320 330 340 350
SYSASLESYA APHQNGYLKA EAVSSYALGG AAFCYWLWRQ QRAGSEQPHG
360 370 380 390 400
SVLSAWGEPD VGYENVLEAE RARREVEHIM LATAPLQAET AVVYSDRAKV
410 420 430 440 450
FLKTEPHRGL HYRTLITEFY DRLLKMGIHR DVILEGSPLD GYKLLFTPFI
460 470 480 490 500
HYLPPAFIKK AEAFAQSGGI WIAGPLTGGR TEHHTIHTDC GLGPLEKCSG
510 520 530 540 550
VKTLFTFPMD ERNSSGTAFG VKAPLSLWSA VFEAGGTKAV GMIEKGPASG
560 570 580 590 600
KAFITEHKCG KGKIVMLGSM PAGEAGDIMM KKLISHYAEE AGVEQKTDVT
610 620 630 640 650
PGTVVAPRKG ADGLVWVVIN MDGKGGAVTL DGNGTDLLSG RPVTGRVTLG
660
PHDYRVILLS ENK
Length:663
Mass (Da):74,214
Last modified:March 24, 2009 - v2
Checksum:iC2044F388107C6C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU40286.1.
CP000002 Genomic DNA. Translation: AAU22933.1.
RefSeqiYP_006712760.1. NC_006322.1.
YP_078571.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU22933; AAU22933; BL03780.
AAU40286; AAU40286; BLi01382.
GeneIDi3030421.
3101523.
KEGGibld:BLi01382.
bli:BL03780.
PATRICi18948341. VBIBacLic203714_1374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU40286.1.
CP000002 Genomic DNA. Translation: AAU22933.1.
RefSeqiYP_006712760.1. NC_006322.1.
YP_078571.1. NC_006270.3.

3D structure databases

ProteinModelPortaliQ65KX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BL03780.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU22933; AAU22933; BL03780.
AAU40286; AAU40286; BLi01382.
GeneIDi3030421.
3101523.
KEGGibld:BLi01382.
bli:BL03780.
PATRICi18948341. VBIBacLic203714_1374.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000095202.
KOiK01190.
OrthoDBiEOG6HMX9F.

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1371-MONOMER.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.

Entry informationi

Entry nameiBGAL1_BACLD
AccessioniPrimary (citable) accession number: Q65KX8
Secondary accession number(s): Q62WC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.