Q65KU8 (PROB1_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase 1
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase 1
Short name=GK 1

Gene names

Name:	proB1
Ordered Locus Names:	BLi01412, BL03752

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Glutamate 5-kinase 1 HAMAP MF_00456		PRO_0000109638
Regions
	Domain	276 – 353	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q65KU8 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 617F00E32A3CD0B2	FASTA	365	39,114
	10 20 30 40 50 60 MKKQRIVIKI GSSSLTNSKG SIDEEKINDH VRAIAALKKE GHEVIFISSG AVAAGFLQLG 70 80 90 100 110 120 YPARPVTLKG KQAAAAVGQS LLMQTYIEHF ADHDIKPAQI LLTRNDFAKR ERYRNAYATV 130 140 150 160 170 180 MELIERGLVP IINENDSVSV EELTFGDNDM LSALVSGLIH ADKLIILTDI NGLYDSNPAE 190 200 210 220 230 240 HPDARRFDYI PEITDELLGC AASAGSKVGT GGMKSKLLAA KTALSLGVNV FIGAGEGDDK 250 260 270 280 290 300 LIQILKGNGD GTYIGQSDLS SVNNHRQWIA FHSPVSGKIT VDEGAELAIT ENGGSLLPAG 310 320 330 340 350 360 VTAISGDFPK GAVVEVYGPN GLAGKGQTLY SAAELEEVKG KRSDEFHHEE GIEVIHRNDW VSIKE

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU40316.1. CP000002 Genomic DNA. Translation: AAU22963.1.
RefSeq	YP_078601.1. NC_006270.3. YP_091009.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65KU8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65KU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000055891; EBBACP00000054422; EBBACG00000055882. EBBACT00000061581; EBBACP00000060014; EBBACG00000061572.
GeneID	3030459. 3098709.
GenomeReviews	Gene locus BLi01412 in contig AE017333_GR. Gene locus BL03752 in contig CP000002_GR.
KEGG	bld:BLi01412. bli:BL03752.
NMPDR	fig|279010.5.peg.866.
PATRIC	18948405. VBIBacLic203714_1406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
GeneTree	EBGT00050000001887.
HOGENOM	HBG507643.
OMA	TFGDNDM.
PhylomeDB	Q65KU8.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI01412-MONOMER. BLIC279010:BL03752-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB1_BACLD

Accession

Primary (citable) accession number: Q65KU8
Secondary accession number(s): Q62W95

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program