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Q65KT8 (METE_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene names
Name:metE
Ordered Locus Names:BLi01422, BL03738
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length762 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7627625-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
PRO_1000017223

Sites

Metal binding6451Zinc By similarity
Metal binding6471Zinc By similarity
Metal binding7301Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65KT8 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 0246F853242AAE3C

FASTA76286,718
        10         20         30         40         50         60 
MTNVKTSSLG FPRIGLNREW KKSLEAYWKG NTDRETFLKE MDEQFLAALQ TQLDQQIDII 

        70         80         90        100        110        120 
PVSDFTMYDH VLDTAVMFNW IPDRFKDIND PLDTYFAMAR GTKDAVSSEM TKWFNTNYHY 

       130        140        150        160        170        180 
IVPEYEKGAQ YRVTRNKPLQ DYQRAKAALG TETKPVILGL YTFVALAKGY EQQDIKDIYN 

       190        200        210        220        230        240 
QMTPLYIQVL KELEQEGVKW VQIDEPALVT ASPEEAAAVK EIYQTITEEV SELNILLQTY 

       250        260        270        280        290        300 
FDSVDAYEEL ISFPVAGIGL DFVHDKGKNF EHLKAHGFPK DKVLAAGILD GRNIWKANLE 

       310        320        330        340        350        360 
ERLDLTLELI QRAGVDEVWI QPSNSLLHVP VAKHPGEHLA DDLLNGLSFA KEKLLELTLL 

       370        380        390        400        410        420 
KNGLVSGKAA IQAEIDEAHG HLQDLKQYGA ATNSAFAEER GKLTEEDFKR PTAFEERLRI 

       430        440        450        460        470        480 
QNDSLGLPLL PTTTIGSFPQ TADVRSARQK WRKKEWSDEQ YEAFIQEETK KWIDIQEDLG 

       490        500        510        520        530        540 
LDVLVHGEFE RTDMVEYFGE KLGGFAFTKY AWVQSYGSRC VRPPVIYGDV EFKEPMTVKE 

       550        560        570        580        590        600 
TVYAQSLTSK KVKGMLTGPV TILNWSFARY DLPRKEIAFQ IACALRKEVE ALEKAGIQII 

       610        620        630        640        650        660 
QVDEPALREG LPLKERDWDE YLKWAAEAFR LSTSSVEDTT QIHTHMCYSN FEDIVDAIED 

       670        680        690        700        710        720 
LDADVITIEH SRSHGGFLDY LEQHPYLKGL GLGVYDIHSP RVPSSDEMLT IIEDALKVCP 

       730        740        750        760 
ADRFWVNPDC GLKTRQPEET IAALKNMVEA AKQARGKLAQ TV

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000002 Genomic DNA. Translation: AAU22973.1.
AE017333 Genomic DNA. Translation: AAU40326.1.
RefSeqYP_078611.1. NC_006270.3.
YP_091019.1. NC_006322.1.

3D structure databases

HSSPHSSP built from PDB template 1XDJ based on UniProtKB Q9X112.
ProteinModelPortalQ65KT8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ65KT8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000056208; EBBACP00000054739; EBBACG00000056199.
EBBACT00000058876; EBBACP00000057309; EBBACG00000058867.
GeneID3030479.
3098755.
GenomeReviewsGene locus BLi01422 in contig AE017333_GR.
Gene locus BL03738 in contig CP000002_GR.
KEGGbld:BLi01422.
bli:BL03738.
NMPDRfig|279010.5.peg.876.
PATRIC18948425. VBIBacLic203714_1416.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0620.
GeneTreeEBGT00050000001686.
HOGENOMHBG287495.
OMARNIWRAN.
PhylomeDBQ65KT8.
ProtClustDBPRK05222.

Enzyme and pathway databases

BioCycBLIC279010-1:BLI01422-MONOMER.
BLIC279010:BL03738-MONOMER.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETE_BACLD
AccessionPrimary (citable) accession number: Q65KT8
Secondary accession number(s): Q62W85
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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