Reviewed, UniProtKB/Swiss-Prot Q65KT8 (METE_BACLD)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BLi01422, BL03738

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017223
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65KT8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 0246F853242AAE3C	FASTA	762	86,718
	10 20 30 40 50 60 MTNVKTSSLG FPRIGLNREW KKSLEAYWKG NTDRETFLKE MDEQFLAALQ TQLDQQIDII 70 80 90 100 110 120 PVSDFTMYDH VLDTAVMFNW IPDRFKDIND PLDTYFAMAR GTKDAVSSEM TKWFNTNYHY 130 140 150 160 170 180 IVPEYEKGAQ YRVTRNKPLQ DYQRAKAALG TETKPVILGL YTFVALAKGY EQQDIKDIYN 190 200 210 220 230 240 QMTPLYIQVL KELEQEGVKW VQIDEPALVT ASPEEAAAVK EIYQTITEEV SELNILLQTY 250 260 270 280 290 300 FDSVDAYEEL ISFPVAGIGL DFVHDKGKNF EHLKAHGFPK DKVLAAGILD GRNIWKANLE 310 320 330 340 350 360 ERLDLTLELI QRAGVDEVWI QPSNSLLHVP VAKHPGEHLA DDLLNGLSFA KEKLLELTLL 370 380 390 400 410 420 KNGLVSGKAA IQAEIDEAHG HLQDLKQYGA ATNSAFAEER GKLTEEDFKR PTAFEERLRI 430 440 450 460 470 480 QNDSLGLPLL PTTTIGSFPQ TADVRSARQK WRKKEWSDEQ YEAFIQEETK KWIDIQEDLG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE KLGGFAFTKY AWVQSYGSRC VRPPVIYGDV EFKEPMTVKE 550 560 570 580 590 600 TVYAQSLTSK KVKGMLTGPV TILNWSFARY DLPRKEIAFQ IACALRKEVE ALEKAGIQII 610 620 630 640 650 660 QVDEPALREG LPLKERDWDE YLKWAAEAFR LSTSSVEDTT QIHTHMCYSN FEDIVDAIED 670 680 690 700 710 720 LDADVITIEH SRSHGGFLDY LEQHPYLKGL GLGVYDIHSP RVPSSDEMLT IIEDALKVCP 730 740 750 760 ADRFWVNPDC GLKTRQPEET IAALKNMVEA AKQARGKLAQ TV

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU22973.1. AE017333 Genomic DNA. Translation: AAU40326.1.
RefSeq	YP_078611.1. YP_091019.1.
3D structure databases
HSSP	HSSP built from PDB template 1XDJ based on UniProtKB Q9X112.
SMR	Q65KT8. Positions 4-757.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65KT8.
Genome annotation databases
GeneID	3030479. 3098755.
GenomeReviews	Gene locus BLi01422 in contig AE017333_GR. Gene locus BL03738 in contig CP000002_GR.
KEGG	bld:BLi01422. bli:BL03738.
NMPDR	fig|279010.5.peg.876.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	SFVRNDI.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI01422-MONOMER. BLIC279010:BL03738-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACLD

Accession

Primary (citable) accession number: Q65KT8
Secondary accession number(s): Q62W85

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)