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Reviewed, UniProtKB/Swiss-Prot Q65KI3 (QUEF_BACLD)

Last modified November 25, 2008. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase
Gene names
Name: queF
Ordered Locus Names: BLi01530, BL03549
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillus

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.

Pathway

tRNA modification; queuosine-tRNA biosynthesis.

Subcellular location

CytoplasmProbable.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165NADPH-dependent 7-cyano-7-deazaguanine reductase
PRO_0000162956

Sequences

Sequence LengthMass (Da)Tools
Q65KI3-1 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 63FF69FC3FDF3FB9

FASTA16519,406
        10         20         30         40         50         60 
MTTRKDSELE GVTLLGNQGT NYLFDYSPEV LESFPNKHEN RDYFVKFNCP EFTSLCPKTG 

        70         80         90        100        110        120 
QPDFATIYIS YIPDKKMVES KSLKLYLFSF RNHGDFHEDC MNIIMNDLIE LMDPRYIEVW 

       130        140        150        160 
GKFTPRGGIS IDPYTNYGKP GTKYEKMAEY RMMNHDLYPE TIDNR 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

AE017333 Genomic DNA. Translation: AAU40431.1. Different initiation.
CP000002 Genomic DNA. Translation: AAU23077.1.
RefSeqYP_078715.1.
YP_091124.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3030614.
3099344.
GenomeReviewsGene locus BL03549 in contig CP000002_GR.
Gene locus BLi01530 in contig AE017333_GR.
KEGGbld:BLi01530.
bli:BL03549.
NMPDRfig|279010.5.peg.1082.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ65KI3.

Enzyme and pathway databases

BioCycBLIC279010:BL03549-MON.

Family and domain databases

HAMAPMF_00818.
[Tree]
InterProIPR016856. CN_OxRdtase_NADPH-dep_QueF.
IPR001474. GTP_CycOHase_I.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsTIGR03139. QueF-II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_BACLD
AccessionPrimary (citable) accession number: Q65KI3
Secondary accession number(s): Q62VY1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 25, 2008
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents