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Q65JY4

- MURE_BACLD

UniProt

Q65JY4 - MURE_BACLD

Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

    Catalytic activityi

    ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei31 – 311UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei150 – 1501UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei178 – 1781UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei186 – 1861UDP-MurNAc-L-Ala-D-GluUniRule annotation
    Binding sitei384 – 3841Meso-diaminopimelateUniRule annotation
    Binding sitei458 – 4581Meso-diaminopimelate; via carbonyl oxygenUniRule annotation
    Binding sitei462 – 4621Meso-diaminopimelateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi109 – 1157ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    4. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBLIC279010:GJ2P-1721-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
    Alternative name(s):
    Meso-A2pm-adding enzymeUniRule annotation
    Meso-diaminopimelate-adding enzymeUniRule annotation
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
    UDP-MurNAc-tripeptide synthetaseUniRule annotation
    UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
    Gene namesi
    Name:murEUniRule annotation
    Ordered Locus Names:BLi01735, BL02239
    OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
    Taxonomic identifieri279010 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligasePRO_1000012339Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei218 – 2181N6-carboxylysineUniRule annotation

    Post-translational modificationi

    Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi279010.BL02239.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65JY4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni151 – 1522UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation
    Regioni408 – 4114Meso-diaminopimelate bindingUniRule annotation

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi408 – 4114Meso-diaminopimelate recognition motif

    Sequence similaritiesi

    Belongs to the MurCDEF family. MurE subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0769.
    HOGENOMiHOG000268118.
    KOiK01928.
    OMAiVDYAHTG.
    OrthoDBiEOG6PKFCR.

    Family and domain databases

    Gene3Di3.40.1190.10. 1 hit.
    3.40.1390.10. 1 hit.
    3.90.190.20. 1 hit.
    HAMAPiMF_00208. MurE.
    InterProiIPR004101. Mur_ligase_C.
    IPR013221. Mur_ligase_cen.
    IPR000713. Mur_ligase_N.
    IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
    [Graphical view]
    PfamiPF01225. Mur_ligase. 1 hit.
    PF02875. Mur_ligase_C. 1 hit.
    PF08245. Mur_ligase_M. 1 hit.
    [Graphical view]
    SUPFAMiSSF53244. SSF53244. 1 hit.
    SSF53623. SSF53623. 1 hit.
    SSF63418. SSF63418. 1 hit.
    TIGRFAMsiTIGR01085. murE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q65JY4-1 [UniParc]FASTAAdd to Basket

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    MKLTKLLTYL KNVPSYAGQE DPDITSIEMD SREVKTGSLF VCIKGYTVDG    50
    HDYARQAAEK GAAAIVAERE VDADVPVIII RHTKRALAVL SDAFYGQPTK 100
    QLRLIGITGT NGKTSTSHMV EEIFRKAGSR TGLIGTMYTK IHDETFEAKN 150
    TTPESVTLQK TFRKMVDQGV DTAVMEVSSH ALHMGRVHGC DYDIAAFTNL 200
    TQDHLDYHET MEEYKHAKSL LFSQLGGSFN HETPKWAVLN ADDPASAYFA 250
    QVTSAHLLTY GIQNDADVMA ENIKMAPKGT TFDLVTPKGS AQVTIPLVGL 300
    FNVYNVLTAA AIAIAADIPF ATITEGIEGL KGVRGRFELV DAGQDFPVIV 350
    DYAHTPDSLE NVLNTCRGLT EGKLFVVVGC GGDRDKTKRP KMAKIAVDLA 400
    DEPVFTADNP RSENPLAILN DMEEGVKGAY YHSIVNREQA IFFAIANAKK 450
    GDVVLIAGKG HETYQQIGGQ TFDFDDAEVA KRAILELK 488
    Length:488
    Mass (Da):53,242
    Last modified:October 25, 2004 - v1
    Checksum:i5503B7E1C159504E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000002 Genomic DNA. Translation: AAU23273.1.
    AE017333 Genomic DNA. Translation: AAU40630.1.
    RefSeqiWP_011197958.1. NC_006322.1.
    YP_006713115.1. NC_006322.1.
    YP_078911.1. NC_006270.3.

    Genome annotation databases

    EnsemblBacteriaiAAU23273; AAU23273; BL02239.
    AAU40630; AAU40630; BLi01735.
    GeneIDi3030883.
    3099138.
    KEGGibld:BLi01735.
    bli:BL02239.
    PATRICi18949069. VBIBacLic203714_1738.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000002 Genomic DNA. Translation: AAU23273.1 .
    AE017333 Genomic DNA. Translation: AAU40630.1 .
    RefSeqi WP_011197958.1. NC_006322.1.
    YP_006713115.1. NC_006322.1.
    YP_078911.1. NC_006270.3.

    3D structure databases

    ProteinModelPortali Q65JY4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 279010.BL02239.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU23273 ; AAU23273 ; BL02239 .
    AAU40630 ; AAU40630 ; BLi01735 .
    GeneIDi 3030883.
    3099138.
    KEGGi bld:BLi01735.
    bli:BL02239.
    PATRICi 18949069. VBIBacLic203714_1738.

    Phylogenomic databases

    eggNOGi COG0769.
    HOGENOMi HOG000268118.
    KOi K01928.
    OMAi VDYAHTG.
    OrthoDBi EOG6PKFCR.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci BLIC279010:GJ2P-1721-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.10. 1 hit.
    3.40.1390.10. 1 hit.
    3.90.190.20. 1 hit.
    HAMAPi MF_00208. MurE.
    InterProi IPR004101. Mur_ligase_C.
    IPR013221. Mur_ligase_cen.
    IPR000713. Mur_ligase_N.
    IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
    [Graphical view ]
    Pfami PF01225. Mur_ligase. 1 hit.
    PF02875. Mur_ligase_C. 1 hit.
    PF08245. Mur_ligase_M. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53244. SSF53244. 1 hit.
    SSF53623. SSF53623. 1 hit.
    SSF63418. SSF63418. 1 hit.
    TIGRFAMsi TIGR01085. murE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
      Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.

    Entry informationi

    Entry nameiMURE_BACLD
    AccessioniPrimary (citable) accession number: Q65JY4
    Secondary accession number(s): Q62VD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3