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Q65JY4

- MURE_BACLD

UniProt

Q65JY4 - MURE_BACLD

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Protein
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Gene
murE, BLi01735, BL02239
Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei31 – 311UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei150 – 1501UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei178 – 1781UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei186 – 1861UDP-MurNAc-L-Ala-D-Glu By similarity
Binding sitei384 – 3841Meso-diaminopimelate By similarity
Binding sitei458 – 4581Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding sitei462 – 4621Meso-diaminopimelate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi109 – 1157ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  4. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1721-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (EC:6.3.2.13)
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene namesi
Name:murE
Ordered Locus Names:BLi01735, BL02239
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation
PRO_1000012339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181N6-carboxylysine By similarity

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi279010.BL02239.

Structurei

3D structure databases

ProteinModelPortaliQ65JY4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni151 – 1522UDP-MurNAc-L-Ala-D-Glu binding By similarity
Regioni408 – 4114Meso-diaminopimelate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi408 – 4114Meso-diaminopimelate recognition motifUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0769.
HOGENOMiHOG000268118.
KOiK01928.
OMAiVDYAHTG.
OrthoDBiEOG6PKFCR.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00208. MurE.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamiPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsiTIGR01085. murE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65JY4-1 [UniParc]FASTAAdd to Basket

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MKLTKLLTYL KNVPSYAGQE DPDITSIEMD SREVKTGSLF VCIKGYTVDG    50
HDYARQAAEK GAAAIVAERE VDADVPVIII RHTKRALAVL SDAFYGQPTK 100
QLRLIGITGT NGKTSTSHMV EEIFRKAGSR TGLIGTMYTK IHDETFEAKN 150
TTPESVTLQK TFRKMVDQGV DTAVMEVSSH ALHMGRVHGC DYDIAAFTNL 200
TQDHLDYHET MEEYKHAKSL LFSQLGGSFN HETPKWAVLN ADDPASAYFA 250
QVTSAHLLTY GIQNDADVMA ENIKMAPKGT TFDLVTPKGS AQVTIPLVGL 300
FNVYNVLTAA AIAIAADIPF ATITEGIEGL KGVRGRFELV DAGQDFPVIV 350
DYAHTPDSLE NVLNTCRGLT EGKLFVVVGC GGDRDKTKRP KMAKIAVDLA 400
DEPVFTADNP RSENPLAILN DMEEGVKGAY YHSIVNREQA IFFAIANAKK 450
GDVVLIAGKG HETYQQIGGQ TFDFDDAEVA KRAILELK 488
Length:488
Mass (Da):53,242
Last modified:October 25, 2004 - v1
Checksum:i5503B7E1C159504E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000002 Genomic DNA. Translation: AAU23273.1.
AE017333 Genomic DNA. Translation: AAU40630.1.
RefSeqiWP_011197958.1. NC_006322.1.
YP_006713115.1. NC_006322.1.
YP_078911.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU23273; AAU23273; BL02239.
AAU40630; AAU40630; BLi01735.
GeneIDi3030883.
3099138.
KEGGibld:BLi01735.
bli:BL02239.
PATRICi18949069. VBIBacLic203714_1738.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000002 Genomic DNA. Translation: AAU23273.1 .
AE017333 Genomic DNA. Translation: AAU40630.1 .
RefSeqi WP_011197958.1. NC_006322.1.
YP_006713115.1. NC_006322.1.
YP_078911.1. NC_006270.3.

3D structure databases

ProteinModelPortali Q65JY4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279010.BL02239.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU23273 ; AAU23273 ; BL02239 .
AAU40630 ; AAU40630 ; BLi01735 .
GeneIDi 3030883.
3099138.
KEGGi bld:BLi01735.
bli:BL02239.
PATRICi 18949069. VBIBacLic203714_1738.

Phylogenomic databases

eggNOGi COG0769.
HOGENOMi HOG000268118.
KOi K01928.
OMAi VDYAHTG.
OrthoDBi EOG6PKFCR.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci BLIC279010:GJ2P-1721-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
HAMAPi MF_00208. MurE.
InterProi IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view ]
Pfami PF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view ]
SUPFAMi SSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsi TIGR01085. murE. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.

Entry informationi

Entry nameiMURE_BACLD
AccessioniPrimary (citable) accession number: Q65JY4
Secondary accession number(s): Q62VD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: September 3, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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