Reviewed, UniProtKB/Swiss-Prot Q65JX9 (MURB_BACLD)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BLi01740, BL02244

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224659
Regions
	Domain	29 – 196	168	FAD-binding PCMH-type
Sites
	Active site	174	1	By similarity
	Active site	225	1	Proton donor By similarity
	Active site	295	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65JX9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FBE18626B21B07A7	FASTA	303	32,810
	10 20 30 40 50 60 MDKVIQELKE LEVGKVLENE PLSNHTTIKI GGPADVLVIP KDIQAVKDTM KVVKKHGVKW 70 80 90 100 110 120 TAIGRGSNLL VLDEGIRGVV IKLGQGLDHM EIDGEQVTVG GGYSVVRLAT GISKKGLSGL 130 140 150 160 170 180 EFAAGIPGSV GGAVYMNAGA HGSDISKVLV KALILFEDGT IEWLTNEEMA FSYRTSILQN 190 200 210 220 230 240 KRPGICLEAV LQLEQKERDQ IVAQMQKNKD YRKETQPVSN PCAGSIFRNP LPEHAGRLVE 250 260 270 280 290 300 EAGLKGHQIG GAKVSEMHGN FIVNAGGATA KDVLDLIAFI QKTIKEKYDI DMHTEVEIIG EKR

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU40635.1. CP000002 Genomic DNA. Translation: AAU23278.1.
RefSeq	YP_078916.1. YP_091328.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65JX9.
Genome annotation databases
GeneID	3030889. 3097817.
GenomeReviews	Gene locus BLi01740 in contig AE017333_GR. Gene locus BL02244 in contig CP000002_GR.
KEGG	bld:BLi01740. bli:BL02244.
NMPDR	fig|279010.5.peg.1587.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65JX9.
OMA	GPADILI.
Enzyme and pathway databases
BioCyc	BLIC279010:BL02244-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BACLD

Accession

Primary (citable) accession number: Q65JX9
Secondary accession number(s): Q62VD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)