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Q65JV7

- SYI_BACLD

UniProt

Q65JV7 - SYI_BACLD

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
    Binding sitei597 – 5971ATPUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi912 – 9121ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBLIC279010:GJ2P-1747-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BLi01762, BL02267
    OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
    Taxonomic identifieri279010 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 922922Isoleucine--tRNA ligasePRO_0000098350Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi279010.BL02267.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65JV7.
    SMRiQ65JV7. Positions 1-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65JV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKDTLLMP KTDFPMRGNL PNREPEIQGK WEDMDIYSLV QKRTEGRPMF    50
    VLHDGPPYAN GDIHMGHALN KVLKDFIVRS RSMSGYHAPY VPGWDTHGLP 100
    IETALTKNKK VKRKEMTVAE FRKLCEEYAW QQIEGQKAQF KRLGVRGDWE 150
    NPYVTLKPEF EAQQIKVFGE MAKKGYIYKG KKPVYWSPSS ESALAEAEIE 200
    YHDKRSPSIY VAFDVKDGKG VLTNGEKIVI WTTTPWTIPA NLGIAVHPEL 250
    EYSVVAAGGA RYVMASALIE SVAKAIGFED HEVVQTVKGK DLEHIVAVHP 300
    LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE 350
    KGHMTSEAPG FEGLFYDQAN KPITEQLEEK GALLKLDFIT HSYPHDWRTK 400
    KPTIFRATAQ WFASIKDFRD ELLEAVKKTK WVPEWGETRL YNMIRDRGDW 450
    CISRQRAWGV PIPVFYAENG EPIITDETIN HVSELFREHG SNIWFEKEAN 500
    ELLPEGFTHP GSPNGKFTKE QDIMDVWFDS GSSHQAVLEE REDLVRPADL 550
    YLEGSDQYRG WFNSSISTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSLG 600
    NVVVPAKIMN QFGADILRLW VASVDYQADV RVSDAILKQV AEVYRKIRNT 650
    FRFLHGNIAD FDPAKDAIAV EDLREVDQYI LIKLNSLIEK VKKAYEEYDF 700
    AVIYHAVHNF CAIELSSFYM DFAKDVVYIE HADHKDRRSM QTVFYETLLA 750
    LVKLIAPILP HTADEMWSHF TFVSEKSVQL TDMPETRDIP NAKETEEKFD 800
    SFMKLRDDVL KALETARNEK IIGKSSVASL TLYPNEEAKA LLSSIKEDVK 850
    QLFIVSELAI GGTEADAPED AQSFETGKIV VAQAEGETCE RCRMVSKEIG 900
    EDPDHPELCP RCAGIVKTYY QN 922
    Length:922
    Mass (Da):104,356
    Last modified:October 25, 2004 - v1
    Checksum:iDFCB33B0F71906B6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017333 Genomic DNA. Translation: AAU40657.1.
    CP000002 Genomic DNA. Translation: AAU23298.1.
    RefSeqiYP_006713139.1. NC_006322.1.
    YP_078936.1. NC_006270.3.

    Genome annotation databases

    EnsemblBacteriaiAAU23298; AAU23298; BL02267.
    AAU40657; AAU40657; BLi01762.
    GeneIDi3030915.
    3100552.
    KEGGibld:BLi01762.
    bli:BL02267.
    PATRICi18949125. VBIBacLic203714_1766.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017333 Genomic DNA. Translation: AAU40657.1 .
    CP000002 Genomic DNA. Translation: AAU23298.1 .
    RefSeqi YP_006713139.1. NC_006322.1.
    YP_078936.1. NC_006270.3.

    3D structure databases

    ProteinModelPortali Q65JV7.
    SMRi Q65JV7. Positions 1-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 279010.BL02267.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU23298 ; AAU23298 ; BL02267 .
    AAU40657 ; AAU40657 ; BLi01762 .
    GeneIDi 3030915.
    3100552.
    KEGGi bld:BLi01762.
    bli:BL02267.
    PATRICi 18949125. VBIBacLic203714_1766.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BLIC279010:GJ2P-1747-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
      Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.

    Entry informationi

    Entry nameiSYI_BACLD
    AccessioniPrimary (citable) accession number: Q65JV7
    Secondary accession number(s): Q62VB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3