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Q65JV7

- SYI_BACLD

UniProt

Q65JV7 - SYI_BACLD

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
Binding sitei597 – 5971ATPUniRule annotation
Metal bindingi889 – 8891ZincUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1747-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BLi01762, BL02267
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922Isoleucine--tRNA ligasePRO_0000098350Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BL02267.

Structurei

3D structure databases

ProteinModelPortaliQ65JV7.
SMRiQ65JV7. Positions 1-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65JV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTLLMP KTDFPMRGNL PNREPEIQGK WEDMDIYSLV QKRTEGRPMF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KVLKDFIVRS RSMSGYHAPY VPGWDTHGLP
110 120 130 140 150
IETALTKNKK VKRKEMTVAE FRKLCEEYAW QQIEGQKAQF KRLGVRGDWE
160 170 180 190 200
NPYVTLKPEF EAQQIKVFGE MAKKGYIYKG KKPVYWSPSS ESALAEAEIE
210 220 230 240 250
YHDKRSPSIY VAFDVKDGKG VLTNGEKIVI WTTTPWTIPA NLGIAVHPEL
260 270 280 290 300
EYSVVAAGGA RYVMASALIE SVAKAIGFED HEVVQTVKGK DLEHIVAVHP
310 320 330 340 350
LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE
360 370 380 390 400
KGHMTSEAPG FEGLFYDQAN KPITEQLEEK GALLKLDFIT HSYPHDWRTK
410 420 430 440 450
KPTIFRATAQ WFASIKDFRD ELLEAVKKTK WVPEWGETRL YNMIRDRGDW
460 470 480 490 500
CISRQRAWGV PIPVFYAENG EPIITDETIN HVSELFREHG SNIWFEKEAN
510 520 530 540 550
ELLPEGFTHP GSPNGKFTKE QDIMDVWFDS GSSHQAVLEE REDLVRPADL
560 570 580 590 600
YLEGSDQYRG WFNSSISTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSLG
610 620 630 640 650
NVVVPAKIMN QFGADILRLW VASVDYQADV RVSDAILKQV AEVYRKIRNT
660 670 680 690 700
FRFLHGNIAD FDPAKDAIAV EDLREVDQYI LIKLNSLIEK VKKAYEEYDF
710 720 730 740 750
AVIYHAVHNF CAIELSSFYM DFAKDVVYIE HADHKDRRSM QTVFYETLLA
760 770 780 790 800
LVKLIAPILP HTADEMWSHF TFVSEKSVQL TDMPETRDIP NAKETEEKFD
810 820 830 840 850
SFMKLRDDVL KALETARNEK IIGKSSVASL TLYPNEEAKA LLSSIKEDVK
860 870 880 890 900
QLFIVSELAI GGTEADAPED AQSFETGKIV VAQAEGETCE RCRMVSKEIG
910 920
EDPDHPELCP RCAGIVKTYY QN
Length:922
Mass (Da):104,356
Last modified:October 25, 2004 - v1
Checksum:iDFCB33B0F71906B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU40657.1.
CP000002 Genomic DNA. Translation: AAU23298.1.
RefSeqiYP_006713139.1. NC_006322.1.
YP_078936.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU23298; AAU23298; BL02267.
AAU40657; AAU40657; BLi01762.
GeneIDi3030915.
3100552.
KEGGibld:BLi01762.
bli:BL02267.
PATRICi18949125. VBIBacLic203714_1766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU40657.1 .
CP000002 Genomic DNA. Translation: AAU23298.1 .
RefSeqi YP_006713139.1. NC_006322.1.
YP_078936.1. NC_006270.3.

3D structure databases

ProteinModelPortali Q65JV7.
SMRi Q65JV7. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279010.BL02267.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU23298 ; AAU23298 ; BL02267 .
AAU40657 ; AAU40657 ; BLi01762 .
GeneIDi 3030915.
3100552.
KEGGi bld:BLi01762.
bli:BL02267.
PATRICi 18949125. VBIBacLic203714_1766.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BLIC279010:GJ2P-1747-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.

Entry informationi

Entry nameiSYI_BACLD
AccessioniPrimary (citable) accession number: Q65JV7
Secondary accession number(s): Q62VB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3