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Q65JV7

- SYI_BACLD

UniProt

Q65JV7 - SYI_BACLD

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BLi01762, BL02267
Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi892 – 8921Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi912 – 9121Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1747-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BLi01762, BL02267
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098350Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BL02267.

Structurei

3D structure databases

ProteinModelPortaliQ65JV7.
SMRiQ65JV7. Positions 1-918.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65JV7-1 [UniParc]FASTAAdd to Basket

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MDYKDTLLMP KTDFPMRGNL PNREPEIQGK WEDMDIYSLV QKRTEGRPMF    50
VLHDGPPYAN GDIHMGHALN KVLKDFIVRS RSMSGYHAPY VPGWDTHGLP 100
IETALTKNKK VKRKEMTVAE FRKLCEEYAW QQIEGQKAQF KRLGVRGDWE 150
NPYVTLKPEF EAQQIKVFGE MAKKGYIYKG KKPVYWSPSS ESALAEAEIE 200
YHDKRSPSIY VAFDVKDGKG VLTNGEKIVI WTTTPWTIPA NLGIAVHPEL 250
EYSVVAAGGA RYVMASALIE SVAKAIGFED HEVVQTVKGK DLEHIVAVHP 300
LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE 350
KGHMTSEAPG FEGLFYDQAN KPITEQLEEK GALLKLDFIT HSYPHDWRTK 400
KPTIFRATAQ WFASIKDFRD ELLEAVKKTK WVPEWGETRL YNMIRDRGDW 450
CISRQRAWGV PIPVFYAENG EPIITDETIN HVSELFREHG SNIWFEKEAN 500
ELLPEGFTHP GSPNGKFTKE QDIMDVWFDS GSSHQAVLEE REDLVRPADL 550
YLEGSDQYRG WFNSSISTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSLG 600
NVVVPAKIMN QFGADILRLW VASVDYQADV RVSDAILKQV AEVYRKIRNT 650
FRFLHGNIAD FDPAKDAIAV EDLREVDQYI LIKLNSLIEK VKKAYEEYDF 700
AVIYHAVHNF CAIELSSFYM DFAKDVVYIE HADHKDRRSM QTVFYETLLA 750
LVKLIAPILP HTADEMWSHF TFVSEKSVQL TDMPETRDIP NAKETEEKFD 800
SFMKLRDDVL KALETARNEK IIGKSSVASL TLYPNEEAKA LLSSIKEDVK 850
QLFIVSELAI GGTEADAPED AQSFETGKIV VAQAEGETCE RCRMVSKEIG 900
EDPDHPELCP RCAGIVKTYY QN 922
Length:922
Mass (Da):104,356
Last modified:October 25, 2004 - v1
Checksum:iDFCB33B0F71906B6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017333 Genomic DNA. Translation: AAU40657.1.
CP000002 Genomic DNA. Translation: AAU23298.1.
RefSeqiYP_006713139.1. NC_006322.1.
YP_078936.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU23298; AAU23298; BL02267.
AAU40657; AAU40657; BLi01762.
GeneIDi3030915.
3100552.
KEGGibld:BLi01762.
bli:BL02267.
PATRICi18949125. VBIBacLic203714_1766.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017333 Genomic DNA. Translation: AAU40657.1 .
CP000002 Genomic DNA. Translation: AAU23298.1 .
RefSeqi YP_006713139.1. NC_006322.1.
YP_078936.1. NC_006270.3.

3D structure databases

ProteinModelPortali Q65JV7.
SMRi Q65JV7. Positions 1-918.
ModBasei Search...

Protein-protein interaction databases

STRINGi 279010.BL02267.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU23298 ; AAU23298 ; BL02267 .
AAU40657 ; AAU40657 ; BLi01762 .
GeneIDi 3030915.
3100552.
KEGGi bld:BLi01762.
bli:BL02267.
PATRICi 18949125. VBIBacLic203714_1766.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BLIC279010:GJ2P-1747-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.

Entry informationi

Entry nameiSYI_BACLD
AccessioniPrimary (citable) accession number: Q65JV7
Secondary accession number(s): Q62VB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi