Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi889ZincUniRule annotation1
Metal bindingi892ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi912ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BLi01762, BL02267
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983501 – 922Isoleucine--tRNA ligaseAdd BLAST922

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi01762

Structurei

3D structure databases

ProteinModelPortaliQ65JV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q65JV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKDTLLMP KTDFPMRGNL PNREPEIQGK WEDMDIYSLV QKRTEGRPMF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KVLKDFIVRS RSMSGYHAPY VPGWDTHGLP
110 120 130 140 150
IETALTKNKK VKRKEMTVAE FRKLCEEYAW QQIEGQKAQF KRLGVRGDWE
160 170 180 190 200
NPYVTLKPEF EAQQIKVFGE MAKKGYIYKG KKPVYWSPSS ESALAEAEIE
210 220 230 240 250
YHDKRSPSIY VAFDVKDGKG VLTNGEKIVI WTTTPWTIPA NLGIAVHPEL
260 270 280 290 300
EYSVVAAGGA RYVMASALIE SVAKAIGFED HEVVQTVKGK DLEHIVAVHP
310 320 330 340 350
LYGRDSLVML GEHVTTDAGT GCVHTAPGHG EDDFIIGQKY GLDVLCPVDE
360 370 380 390 400
KGHMTSEAPG FEGLFYDQAN KPITEQLEEK GALLKLDFIT HSYPHDWRTK
410 420 430 440 450
KPTIFRATAQ WFASIKDFRD ELLEAVKKTK WVPEWGETRL YNMIRDRGDW
460 470 480 490 500
CISRQRAWGV PIPVFYAENG EPIITDETIN HVSELFREHG SNIWFEKEAN
510 520 530 540 550
ELLPEGFTHP GSPNGKFTKE QDIMDVWFDS GSSHQAVLEE REDLVRPADL
560 570 580 590 600
YLEGSDQYRG WFNSSISTAV AVTGKAPYKG VLSHGFALDG EGRKMSKSLG
610 620 630 640 650
NVVVPAKIMN QFGADILRLW VASVDYQADV RVSDAILKQV AEVYRKIRNT
660 670 680 690 700
FRFLHGNIAD FDPAKDAIAV EDLREVDQYI LIKLNSLIEK VKKAYEEYDF
710 720 730 740 750
AVIYHAVHNF CAIELSSFYM DFAKDVVYIE HADHKDRRSM QTVFYETLLA
760 770 780 790 800
LVKLIAPILP HTADEMWSHF TFVSEKSVQL TDMPETRDIP NAKETEEKFD
810 820 830 840 850
SFMKLRDDVL KALETARNEK IIGKSSVASL TLYPNEEAKA LLSSIKEDVK
860 870 880 890 900
QLFIVSELAI GGTEADAPED AQSFETGKIV VAQAEGETCE RCRMVSKEIG
910 920
EDPDHPELCP RCAGIVKTYY QN
Length:922
Mass (Da):104,356
Last modified:October 25, 2004 - v1
Checksum:iDFCB33B0F71906B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA Translation: AAU40657.1
CP000002 Genomic DNA Translation: AAU23298.1
RefSeqiWP_009328543.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU23298; AAU23298; BL02267
AAU40657; AAU40657; BLi01762
GeneIDi3030915
KEGGibld:BLi01762
bli:BL02267

Similar proteinsi

Entry informationi

Entry nameiSYI_BACLD
AccessioniPrimary (citable) accession number: Q65JV7
Secondary accession number(s): Q62VB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 25, 2004
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health