Q65JT0 (KGUA_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	gmk
Ordered Locus Names:	BLi01789, BL02293

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus licheniformis

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP By similarity. HAMAP-Rule MF_00328
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP-Rule MF_00328
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00328.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Guanylate kinase HAMAP-Rule MF_00328		PRO_0000266288
Regions
	Domain	5 – 184	180	Guanylate kinase-like
	Nucleotide binding	12 – 19	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65JT0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: D39E17632B99BCBC	FASTA	204	23,469
	10 20 30 40 50 60 MKERGLLIVL SGPSGVGKGT VRQALFAQED TKFEYSISVT TRKPRQGERD GVDYFFKTRE 70 80 90 100 110 120 EFEEMIENNK LLEWAEYVGN YYGTPVDYVE QTLESGRDVF LEIEVQGALQ VRKAFPEGLF 130 140 150 160 170 180 IFLAPPSLAE LKNRIVTRGT ETEATIENRM KAAKEEIELM DAYDYVVEND SIELACERIK 190 200 AIVLAEHLRR DRVAPRYKKM LEVE

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU40684.1. CP000002 Genomic DNA. Translation: AAU23324.2.
RefSeq	YP_006713165.1. NC_006322.1. YP_078962.2. NC_006270.3.
3D structure databases
ProteinModelPortal	Q65JT0.
ModBase	Search...
Protein-protein interaction databases
STRING	279010.BL02293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU23324; AAU23324; BL02293. AAU40684; AAU40684; BLi01789.
GeneID	3030944. 3098229.
KEGG	bld:BLi01789. bli:BL02293.
PATRIC	18949179. VBIBacLic203714_1793.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0194.
HOGENOM	HOG000037639.
KO	K00942.
OMA	FVIINEL.
ProtClustDB	PRK00300.
Enzyme and pathway databases
BioCyc	BLIC279010:GJ2P-1774-MONOMER.
Family and domain databases
HAMAP	MF_00328. Guanylate_kinase.
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_BACLD

Accession

Primary (citable) accession number: Q65JT0
Secondary accession number(s): Q62V85

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 25, 2004
Last modified:	May 29, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program