Reviewed, UniProtKB/Swiss-Prot Q65JQ5 (RNC_BACLD)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	BLi01814, BL02317

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: HAMAP rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Ribonuclease 3 HAMAP MF_00104		PRO_0000228494
Regions
	Domain	20 – 149	130	RNase III
	Domain	175 – 244	70	DRBM

Sequences

	Sequence		Length	Mass (Da)
	Q65JQ5-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 8EC3EA613B4FAD28	FASTA	249	28,385
	10 20 30 40 50 60 MSKHSHFRDK KMFQKKAEQF KKFQERISVQ FQNEKLLYQA FTHSSYVNEH RKKPYEDNER 70 80 90 100 110 120 LEFLGDAVLE LTISQFLYAK YPAMSEGDLT KLRAAIVCEP SLVSLAHELS FGELVLLGKG 130 140 150 160 170 180 EEMTGGRKRP ALLADVFEAF IGALYLDQGL EAVVTFLKAY VFPKIDDGAF SHVMDFKSQL 190 200 210 220 230 240 QEFVQRDGRG ILEYKILQEK GPAHNREFEA NVSLKGEVLG VGSGRSKKEA EQHAAQEALA KLQKHHMKQ

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU40709.1. CP000002 Genomic DNA. Translation: AAU23349.2.
RefSeq	YP_078987.2. YP_091402.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65JQ5.
Genome annotation databases
GeneID	3030981. 3100412.
GenomeReviews	Gene locus BLi01814 in contig AE017333_GR. Gene locus BL02317 in contig CP000002_GR.
KEGG	bld:BLi01814. bli:BL02317.
NMPDR	fig|279010.5.peg.2898.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65JQ5.
Enzyme and pathway databases
BioCyc	BLIC279010:BL02317-MON.
Family and domain databases
HAMAP	MF_00104. [Tree]
InterPro	IPR001159. Ds-RNA_bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BACLD

Accession

Primary (citable) accession number: Q65JQ5
Secondary accession number(s): Q62V60

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	October 25, 2004
Last modified:	September 1, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)