Reviewed, UniProtKB/Swiss-Prot Q65JP6 (TRMD_BACLD)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BLi01823, BL01291

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060325
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	113	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65JP6-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: C91ECE84008616C5	FASTA	243	27,825
	10 20 30 40 50 60 MKIDFLTLFP EMFEGVLGSS ILQKAQDKDA VRFRVVNFRA YSDNKHQTVD DYPYGGGAGM 70 80 90 100 110 120 VLKPQPVFDA VEKLTAEAGG SPRIILVCPQ GERYTQQKAE ELAREEHLMF ICGHYEGYDE 130 140 150 160 170 180 RIREHLSTDE ISIGDFVLTG GELPAMMIAD SVVRLLPGVL GKEESHLEDS FSTGLLEHPH 190 200 210 220 230 240 YTRPADYKGL KVPDVLTSGN HAKIKEWRRK ESLRRTFLRR PDLLENYPLS EEERKWISEW KNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Berka R.M., Rey M.W., Ramaiya P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU40718.1. CP000002 Genomic DNA. Translation: AAU23358.2.
RefSeq	YP_078996.2. YP_091411.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65JP6.
Genome annotation databases
GeneID	3030991. 3097986.
GenomeReviews	Gene locus BLi01823 in contig AE017333_GR. Gene locus BL01291 in contig CP000002_GR.
KEGG	bld:BLi01823. bli:BL01291.
NMPDR	fig|279010.5.peg.2527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65JP6.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01291-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BACLD

Accession

Primary (citable) accession number: Q65JP6
Secondary accession number(s): Q62V51

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 25, 2004
Last modified:	September 1, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)