Q65JN7 (Q65JN7_BACLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL AAU40727.1
Ordered Locus Names:	BL01281, BLi01832

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733 RuleBase RU000518
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q65JN7 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: D9CF5A50C33F441B	FASTA	691	79,174
	10 20 30 40 50 60 MSEYLVIVES PAKAKTIERY LGKKYKVKAS MGHVRDLPKS QMGVDIEQNF EPKYITIRGK 70 80 90 100 110 120 GPVLKELKTA AKKAKKVYLA ADPDREGEAI AWHLAHSLDL DLNSDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRMINM DLVDAQQARR ILDRLVGYKI SPILWKKVKK GLSAGRVQSV ALRLIIDREK 190 200 210 220 230 240 EINDFIPEEY WTIEGSFLKG KETFEASFFG INGEKHQLKS EDDVKQILSR IKGNKFEVKK 250 260 270 280 290 300 VTKKERKRNP ALPFTTSTLQ QEAARKLNFR AKKTMMIAQQ LYEGIDLGKE GTVGLITYMR 310 320 330 340 350 360 TDSTRISNTA QEEAASFIGE QYGKEFLGSK RKPAKKNENA QDAHEAIRPT SVLRKPSDLK 370 380 390 400 410 420 AVLGRDQLRL YKLIWERFVA SQMAPATLDT MSVDLENNGL TFRANGSKVK FSGFMKVYVE 430 440 450 460 470 480 GKDDQMEEKN KMLPDLAEGD TVLSKDIEPE QHFTQPPPRY TEARLVKTLE ELGIGRPSTY 490 500 510 520 530 540 APTLDTIQKR GYVALDNKRF IPTELGEIVL DLIMEFFPEI INVEFTAKME KELDDVEDGN 550 560 570 580 590 600 IQWVQIIDSF YKDFEKRVEK AEAEMQEVEI EPEYAGVDCE ACGHPMVYKM GRYGKFMACS 610 620 630 640 650 660 NFPDCRNTKP IVKEIGVKCP SCKTGNIVER KSKKRRIFYG CDRYPECEFV SWDKPLERKC 670 680 690 PKCEDMLVEK KLKKGVQVQC VNCDYKEEQQ K

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580 [Goettingen].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580 [Novozymes].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU23367.2. AE017333 Genomic DNA. Translation: AAU40727.1.
RefSeq	YP_079005.2. NC_006270.3. YP_091420.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65JN7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65JN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000056899; EBBACP00000055430; EBBACG00000056890. EBBACT00000061629; EBBACP00000060062; EBBACG00000061620.
GeneID	3031004. 3098695.
GenomeReviews	Gene locus BLi01832 in contig AE017333_GR. Gene locus BL01281 in contig CP000002_GR.
KEGG	bld:BLi01832. bli:BL01281.
NMPDR	fig|279010.5.peg.2535.
PATRIC	18949263. VBIBacLic203714_1835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00070000032133.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q65JN7_BACLD

Accession

Primary (citable) accession number: Q65JN7
Secondary accession number(s): Q62V42

Entry history

Integrated into UniProtKB/TrEMBL:	October 25, 2004
Last sequence update:	October 25, 2004
Last modified:	December 14, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)