Reviewed, UniProtKB/Swiss-Prot Q65JJ1 (SYP_BACLD)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BLi01878, BL01235

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000248648

Sequences

	Sequence		Length	Mass (Da)
	Q65JJ1-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 5F38EB6AC80A6ECD	FASTA	572	64,138
	10 20 30 40 50 60 MRQSRTLIPT LREVPADAEA KSHQLLLRAG FIRQNTSGVY SYMPLANKVI HKIQSIVREE 70 80 90 100 110 120 MEKINAVEML MPALQQAETW QESGRWYTYG PELMRLKDRH GREFALGATH EEVITSIVRD 130 140 150 160 170 180 EVKSYKRLPL TLYQIQSKFR DEKRPRFGLL RGREFIMKDA YSFHSSAESL DETYNDMYQA 190 200 210 220 230 240 YTNVFTRCGL NFRPVIADSG AMGGKDTHEF MALSDVGEDT IAYSDQSSYA ANIEMAEVKE 250 260 270 280 290 300 TDAGEQAEMK ELQEVHTPSV KTIEEVAAFL GISPSDCIKS MLMKADGRFV LVLTRGDHEV 310 320 330 340 350 360 NDVKVKNLLQ AEIIEFASAE EVAEITGTEP GFVGPVGLDR EIEIFADFAV KAMANAAAGA 370 380 390 400 410 420 NKTDYHYQNV NISRDAHNVT FADLRFIQEG DPSPDGKGTI RFAKGIEVGQ VFKLGTRYSE 430 440 450 460 470 480 AMDATYLDEN GRAQPMLMGC YGIGISRTLS AIVEQHHDDK GLIWPLEVTP YDLHILALNM 490 500 510 520 530 540 KNDAQVQLAE KLYEEFKANG YDVLFDDRAE RAGVKFADSD LIGLPIRITV GKRADEGVVE 550 560 570 VKIRKTGESF EIAADELFDF IEKQVKSLSS HS

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU40773.1. CP000002 Genomic DNA. Translation: AAU23413.1.
RefSeq	YP_079051.1. YP_091466.1.
3D structure databases
SMR	Q65JJ1. Positions 1-563.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65JJ1.
Genome annotation databases
GeneID	3031073. 3101626.
GenomeReviews	Gene locus BLi01878 in contig AE017333_GR. Gene locus BL01235 in contig CP000002_GR.
KEGG	bld:BLi01878. bli:BL01235.
NMPDR	fig|279010.5.peg.2582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	DFVLGPT.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI01878-MONOMER. BLIC279010:BL01235-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACLD

Accession

Primary (citable) accession number: Q65JJ1
Secondary accession number(s): Q62UZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)