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Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA1

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotationSAAS annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei45 – 451Part of a proton relay during catalysisUniRule annotation
Binding sitei46 – 461PyruvateUniRule annotation
Sitei108 – 1081Part of a proton relay during catalysisUniRule annotation
Active sitei134 – 1341Proton donor/acceptorUniRule annotation
Active sitei162 – 1621Schiff-base intermediate with substrateUniRule annotation
Binding sitei204 – 2041Pyruvate; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotation, Lysine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

Schiff baseUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1887-MONOMER.
MetaCyc:MONOMER-6565.
UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotationSAAS annotation (EC:4.3.3.7UniRule annotationSAAS annotation)
Short name:
HTPA synthaseUniRule annotation
Gene namesi
Name:dapA1Imported
Synonyms:dapAUniRule annotationImported
Ordered Locus Names:BL01208Imported, BLi01902Imported
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)Imported
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000608 Componenti: Chromosome UP000000606 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BL01208.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiNVIPQEF.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Schiff_base-form_aldolases_AS.
IPR020624. Schiff_base-form_aldolases_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65JG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFGNIATAM VTPFDKNENI DFQKLSKLID YLLNNGTDSL VVAGTTGESP
60 70 80 90 100
TLSEEEKVAL IQYSVKEAAG RAPIIAGTGS NNTKASIKLT KKAEEAGADA
110 120 130 140 150
VMLVTPYYNK PSQEGMYRHF RAIAEETSLP VMLYNVPGRT AASLAPETTI
160 170 180 190 200
RLAEIPNIIA IKEASGDLDA ITKIVAETPE DFAVYSGDDS LTLPALSVGA
210 220 230 240 250
RGIVSVASHI IGPEMQEMIK HYTEGNTAQA ALIHQKLLPL MKGLFAAPNP
260 270 280
SPLKTALQLK GLDVGSVRLP LIPLNEDERL RLSSLMNGL
Length:289
Mass (Da):30,917
Last modified:October 25, 2004 - v1
Checksum:i56AC182E3E53BEE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU23437.2.
AE017333 Genomic DNA. Translation: AAU40797.1.
RefSeqiYP_006713278.1. NC_006322.1.
YP_079075.2. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU23437; AAU23437; BL01208.
AAU40797; AAU40797; BLi01902.
KEGGibld:BLi01902.
bli:BL01208.
PATRICi18949407. VBIBacLic203714_1907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU23437.2.
AE017333 Genomic DNA. Translation: AAU40797.1.
RefSeqiYP_006713278.1. NC_006322.1.
YP_079075.2. NC_006270.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BL01208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU23437; AAU23437; BL01208.
AAU40797; AAU40797; BLi01902.
KEGGibld:BLi01902.
bli:BL01208.
PATRICi18949407. VBIBacLic203714_1907.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiNVIPQEF.
OrthoDBiEOG6W7235.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00017.
BioCyciBLIC279010:GJ2P-1887-MONOMER.
MetaCyc:MONOMER-6565.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Schiff_base-form_aldolases_AS.
IPR020624. Schiff_base-form_aldolases_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580Imported and DSM 13 / ATCC 14580 [Novozymes]Imported.
  2. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13Imported and DSM 13 / ATCC 14580 [Goettingen]Imported.
  3. Berka R.M., Rey M.W., Ramaiya P.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 14580.
  4. Wiegand S., Hertel R., Dietrich S., Volland S., Liesegang H.
    Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 13Imported.

Entry informationi

Entry nameiQ65JG7_BACLD
AccessioniPrimary (citable) accession number: Q65JG7
Secondary accession number(s): Q62UX2
Entry historyi
Integrated into UniProtKB/TrEMBL: October 25, 2004
Last sequence update: October 25, 2004
Last modified: April 29, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.