SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q65JG7

- Q65JG7_BACLD

UniProt

Q65JG7 - Q65JG7_BACLD

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene
dapA1, dapA, BL01208, BLi01902
Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA) By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei45 – 451Part of a proton relay during catalysis By similarityUniRule annotation
Binding sitei46 – 461Pyruvate By similarityUniRule annotation
Sitei108 – 1081Part of a proton relay during catalysis By similarityUniRule annotation
Active sitei134 – 1341Proton donor/acceptor By similarityUniRule annotation
Active sitei162 – 1621Schiff-base intermediate with substrate By similarityUniRule annotation
Binding sitei204 – 2041Pyruvate; via carbonyl oxygen By similarityUniRule annotation

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
  2. amine-lyase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotationsImported

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotationSAAS annotations, Lysine biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

Schiff baseUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-1887-MONOMER.
MetaCyc:MONOMER-6565.
UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
Short name:
HTPA synthaseUniRule annotation
Gene namesi
Name:dapA1Imported
Synonyms:dapAUniRule annotationImported
Ordered Locus Names:BL01208Imported, BLi01902Imported
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)Imported
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer; dimer of dimers By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BL01208.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiMLYSGDD.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65JG7-1 [UniParc]FASTAAdd to Basket

« Hide

MNFGNIATAM VTPFDKNENI DFQKLSKLID YLLNNGTDSL VVAGTTGESP    50
TLSEEEKVAL IQYSVKEAAG RAPIIAGTGS NNTKASIKLT KKAEEAGADA 100
VMLVTPYYNK PSQEGMYRHF RAIAEETSLP VMLYNVPGRT AASLAPETTI 150
RLAEIPNIIA IKEASGDLDA ITKIVAETPE DFAVYSGDDS LTLPALSVGA 200
RGIVSVASHI IGPEMQEMIK HYTEGNTAQA ALIHQKLLPL MKGLFAAPNP 250
SPLKTALQLK GLDVGSVRLP LIPLNEDERL RLSSLMNGL 289
Length:289
Mass (Da):30,917
Last modified:October 25, 2004 - v1
Checksum:i56AC182E3E53BEE5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000002 Genomic DNA. Translation: AAU23437.2.
AE017333 Genomic DNA. Translation: AAU40797.1.
RefSeqiYP_006713278.1. NC_006322.1.
YP_079075.2. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU23437; AAU23437; BL01208.
AAU40797; AAU40797; BLi01902.
GeneIDi3031112.
3098811.
KEGGibld:BLi01902.
bli:BL01208.
PATRICi18949407. VBIBacLic203714_1907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000002 Genomic DNA. Translation: AAU23437.2 .
AE017333 Genomic DNA. Translation: AAU40797.1 .
RefSeqi YP_006713278.1. NC_006322.1.
YP_079075.2. NC_006270.3.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279010.BL01208.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAU23437 ; AAU23437 ; BL01208 .
AAU40797 ; AAU40797 ; BLi01902 .
GeneIDi 3031112.
3098811.
KEGGi bld:BLi01902.
bli:BL01208.
PATRICi 18949407. VBIBacLic203714_1907.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173604.
KOi K01714.
OMAi MLYSGDD.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00017 .
BioCyci BLIC279010:GJ2P-1887-MONOMER.
MetaCyc:MONOMER-6565.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00418. DapA.
InterProi IPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
TIGRFAMsi TIGR00674. dapA. 1 hit.
PROSITEi PS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580Imported and DSM 13 / ATCC 14580 [Novozymes].
  2. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13Imported and DSM 13 / ATCC 14580 [Goettingen].
  3. Berka R.M., Rey M.W., Ramaiya P.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 14580.
  4. Wiegand S., Hertel R., Dietrich S., Volland S., Liesegang H.
    Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 13Imported.

Entry informationi

Entry nameiQ65JG7_BACLD
AccessioniPrimary (citable) accession number: Q65JG7
Secondary accession number(s): Q62UX2
Entry historyi
Integrated into UniProtKB/TrEMBL: October 25, 2004
Last sequence update: October 25, 2004
Last modified: September 3, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi