Reviewed, UniProtKB/Swiss-Prot Q65J44 (TYSY_BACLD)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BLi02029, BL02642

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Thymidylate synthase HAMAP MF_00008		PRO_0000140926
Sites
	Active site	161	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65J44-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 479D3E24E452EA8F	FASTA	279	32,760
	10 20 30 40 50 60 MSHYDQQYNA IIQKIIESGI SDEEYQVRTK WDSDGTPAHT LSIMSEKMRF DNSEVPILTT 70 80 90 100 110 120 KKVAWKTAIK ELLWIWQLKS NDVQVLNDMG VHIWDQWRLE DGTIGAAYGY QLGKKNRTVN 130 140 150 160 170 180 GQKVDQVDYL LHQLKHNPSS RRHLTMLWNP DDLDGMALTP CVYETQWYVK EGKLSLEVRA 190 200 210 220 230 240 RSNDMALGNP FNVFQYNVLQ RMIAQVLGYE LGEYIFNIGD CHIYTRHIDN LNIQMKREQY 250 260 270 EAPKLWINPD IKNFYDFTID DFKLIDYKHG DKLTFEVAV

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU40920.1. CP000002 Genomic DNA. Translation: AAU23558.1.
RefSeq	YP_079196.1. YP_091613.1.
3D structure databases
SMR	Q65J44. Positions 1-279.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65J44.
Genome annotation databases
GeneID	3031282. 3099538.
GenomeReviews	Gene locus BLi02029 in contig AE017333_GR. Gene locus BL02642 in contig CP000002_GR.
KEGG	bld:BLi02029. bli:BL02642.
NMPDR	fig|279010.5.peg.2728.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	DIKPEDF.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI02029-MONOMER. BLIC279010:BL02642-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACLD

Accession

Primary (citable) accession number: Q65J44
Secondary accession number(s): Q62UK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)