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Protein

Thymidylate synthase

Gene

thyA

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161NucleophileUniRule annotation1
Binding sitei1845,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei192dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 142dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi181 – 184dUMPUniRule annotation4
Nucleotide bindingi222 – 224dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:BLi02029, BL02642
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409261 – 279Thymidylate synthaseAdd BLAST279

Proteomic databases

PRIDEiQ65J44

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi02029

Structurei

3D structure databases

ProteinModelPortaliQ65J44
SMRiQ65J44
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257898
KOiK00560
OMAiWNEWEVG

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q65J44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHYDQQYNA IIQKIIESGI SDEEYQVRTK WDSDGTPAHT LSIMSEKMRF
60 70 80 90 100
DNSEVPILTT KKVAWKTAIK ELLWIWQLKS NDVQVLNDMG VHIWDQWRLE
110 120 130 140 150
DGTIGAAYGY QLGKKNRTVN GQKVDQVDYL LHQLKHNPSS RRHLTMLWNP
160 170 180 190 200
DDLDGMALTP CVYETQWYVK EGKLSLEVRA RSNDMALGNP FNVFQYNVLQ
210 220 230 240 250
RMIAQVLGYE LGEYIFNIGD CHIYTRHIDN LNIQMKREQY EAPKLWINPD
260 270
IKNFYDFTID DFKLIDYKHG DKLTFEVAV
Length:279
Mass (Da):32,760
Last modified:October 25, 2004 - v1
Checksum:i479D3E24E452EA8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA Translation: AAU40920.1
CP000002 Genomic DNA Translation: AAU23558.1
RefSeqiWP_003182218.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU23558; AAU23558; BL02642
AAU40920; AAU40920; BLi02029
GeneIDi3031282
KEGGibld:BLi02029
bli:BL02642

Similar proteinsi

Entry informationi

Entry nameiTYSY_BACLD
AccessioniPrimary (citable) accession number: Q65J44
Secondary accession number(s): Q62UK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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