Reviewed, UniProtKB/Swiss-Prot Q65IS9 (PROA2_BACLD)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase 2
      Short name=GPR 2
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase 2
    Glutamyl-gamma-semialdehyde dehydrogenase 2
      Short name=GSA dehydrogenase 2

Gene names

Name:	proA2
Ordered Locus Names:	BLi02148, BL01984

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Gamma-glutamyl phosphate reductase 2		PRO_0000189694

Sequences

	Sequence		Length	Mass (Da)
	Q65IS9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 38BBCA4EA7002357	FASTA	423	45,601
	10 20 30 40 50 60 MTATSESISA QIEEKAIKAK TAAKSLRLAT EKEKNEALAA LADHLKQNMS YILTENAKDI 70 80 90 100 110 120 EAGRKKGYDA AYIDRLSLNE DRVLDFADGL LEVAELDDPV GETLSSWTLD NGLKAEKVSV 130 140 150 160 170 180 PLGVIGMIYE ARPNVTVDAT GLALKSGNAI VLKGGSSAIH SNTAIVSVMH QALDSTNIPR 190 200 210 220 230 240 DAVQFIESTD RSATKQLFKM KEHIDVLIPR GGGALIQEVV ENATVPVLET GVGNCHIYID 250 260 270 280 290 300 READPEKAID VMINAKTDRP AVCNALETLI VHEKWLEENS AALINALNEH GIQVHGDEKA 310 320 330 340 350 360 VQMIPGARPA GEADWQNEYL SLDLAVKVAG SLEEACDHIE TYGTKHSEAI ITENPETAKA 370 380 390 400 410 420 FLNTVDAAAL YHNASTRFTD GGALGFGAEI GISTQKLHAR GPMGLKELTT CKYIMRGDGH IRR

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41035.1. CP000002 Genomic DNA. Translation: AAU23673.1.
RefSeq	YP_079311.1. YP_091728.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3031437. 3097479.
GenomeReviews	Gene locus BL01984 in contig CP000002_GR. Gene locus BLi02148 in contig AE017333_GR.
KEGG	bld:BLi02148. bli:BL01984.
NMPDR	fig|279010.5.peg.2844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65IS9.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01984-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA2_BACLD

Accession

Primary (citable) accession number: Q65IS9
Secondary accession number(s): Q62U86

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 25, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)