Reviewed, UniProtKB/Swiss-Prot Q65IS8 (PROB2_BACLD)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase 2
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase 2
      Short name=GK 2

Gene names

Name:	proB2
Ordered Locus Names:	BLi02149, BL01983

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Glutamate 5-kinase 2 HAMAP MF_00456		PRO_0000109639
Regions
	Domain	279 – 355	77	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q65IS8-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 3CBEEF1390AED762	FASTA	373	40,474
	10 20 30 40 50 60 MTSNQQRVVV KVGSSSLTSL HGEISSKKLE KLVEQIVRLK DEGHEVLLVS SGAVAAGYRK 70 80 90 100 110 120 LGFINRPDTL PEKQASASVG QGLLIEAYSK LFLSHGYIAS QILITKSDFS DEKRYNNVRN 130 140 150 160 170 180 TMNVLLKRGI IPIINENDTV TVNRLKFGDN DTLSAKVAGL IDADLLTILS DIDGLYDANP 190 200 210 220 230 240 RKTPDAALVQ EVTEITPEIE ASAGDAGSEV GTGGMKSKLD AFKITMASGI KGFLGRADTP 250 260 270 280 290 300 GILHEAICGT ARGTYFIPNA DSHALNHKKQ WIAFNSGPEG DIVVHNESKE FIIDRKKSLY 310 320 330 340 350 360 PSSIYDINGR FSNGSVVRIL DQEGEEIGLG VVNFSSSQLR KWKKDTNQET AASSQAVVDR 370 EAFVCHLELS VPL

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41036.1. CP000002 Genomic DNA. Translation: AAU23674.1.
RefSeq	YP_079312.1. YP_091729.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65IS8.
Genome annotation databases
GeneID	3031438. 3100671.
GenomeReviews	Gene locus BLi02149 in contig AE017333_GR. Gene locus BL01983 in contig CP000002_GR.
KEGG	bld:BLi02149. bli:BL01983.
NMPDR	fig|279010.5.peg.2845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65IS8.
OMA	KLDAFKI.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01983-MON.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. False negative. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB2_BACLD

Accession

Primary (citable) accession number: Q65IS8
Secondary accession number(s): Q62U85

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)