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Q65IS8 (PROB2_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase 2

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase 2
Short name=GK 2
Gene names
Name:proB2
Ordered Locus Names:BLi02149, BL01983
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 373373Glutamate 5-kinase 2 HAMAP-Rule MF_00456
PRO_0000109639

Regions

Domain279 – 35577PUA
Nucleotide binding170 – 1712ATP By similarity
Nucleotide binding212 – 2187ATP By similarity

Sites

Binding site111ATP By similarity
Binding site511Substrate By similarity
Binding site1381Substrate By similarity
Binding site1501Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65IS8 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 3CBEEF1390AED762

FASTA37340,474
        10         20         30         40         50         60 
MTSNQQRVVV KVGSSSLTSL HGEISSKKLE KLVEQIVRLK DEGHEVLLVS SGAVAAGYRK 

        70         80         90        100        110        120 
LGFINRPDTL PEKQASASVG QGLLIEAYSK LFLSHGYIAS QILITKSDFS DEKRYNNVRN 

       130        140        150        160        170        180 
TMNVLLKRGI IPIINENDTV TVNRLKFGDN DTLSAKVAGL IDADLLTILS DIDGLYDANP 

       190        200        210        220        230        240 
RKTPDAALVQ EVTEITPEIE ASAGDAGSEV GTGGMKSKLD AFKITMASGI KGFLGRADTP 

       250        260        270        280        290        300 
GILHEAICGT ARGTYFIPNA DSHALNHKKQ WIAFNSGPEG DIVVHNESKE FIIDRKKSLY 

       310        320        330        340        350        360 
PSSIYDINGR FSNGSVVRIL DQEGEEIGLG VVNFSSSQLR KWKKDTNQET AASSQAVVDR 

       370 
EAFVCHLELS VPL 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017333 Genomic DNA. Translation: AAU41036.1.
CP000002 Genomic DNA. Translation: AAU23674.1.
RefSeqYP_006713514.1. NC_006322.1.
YP_079312.1. NC_006270.3.

3D structure databases

ProteinModelPortalQ65IS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL01983.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU23674; AAU23674; BL01983.
AAU41036; AAU41036; BLi02149.
GeneID3031438.
3100671.
KEGGbld:BLi02149.
bli:BL01983.
PATRIC18949915. VBIBacLic203714_2161.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246369.
KOK00931.
OMAFNSGPEG.
OrthoDBEOG6PGK7G.
ProtClustDBPRK13402.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-2132-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB2_BACLD
AccessionPrimary (citable) accession number: Q65IS8
Secondary accession number(s): Q62U85
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: October 25, 2004
Last modified: February 19, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways