Reviewed, UniProtKB/Swiss-Prot Q65IH4 (ODO1_BACLD)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	BLi02260, BL01452

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	944 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 944	944	2-oxoglutarate dehydrogenase E1 component		PRO_0000162167

Sequences

	Sequence		Length	Mass (Da)
	Q65IH4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 9E63C701C8847E39	FASTA	944	106,671
	10 20 30 40 50 60 MFQNSMKQRM TWEEFHGPNL GYVLELYDQY VKDPESLDAD LKEMFDELGA PPGDIRAASQ 70 80 90 100 110 120 KNEEADFTAG SIQKIASAVK LAEDIRTYGH LNASVNPLRK TQEKQELFPL AEYGLTEQDV 130 140 150 160 170 180 KKIPASVICK DAPKEVTNGL EAIQYLRNTY KKSISFEFDH VHIFEERNWL MKKIESGELF 190 200 210 220 230 240 TPKSKEKLVE VLRRLTEVES LEQFLHKTFV GQKRFSIEGL DALVPMLDDI IAKSVSAGTT 250 260 270 280 290 300 NVNIGMAHRG RLNVLAHVLG KPYEIIFSEF QHAPNKDLVP SEGSTGINYG WTGDVKYHLG 310 320 330 340 350 360 ANRQIQDEHT KTARIALANN PSHLEFIDPI VEGSTRAAQE TRTESGYPVQ DVKKSMAILI 370 380 390 400 410 420 HGDAAFPGEG IVAETLNLSQ LKGYQVGGAI HIIANNMIGF TTESNESRST KYASDLAKGF 430 440 450 460 470 480 EIPIVHVNAD DPEACLSAVQ LAVEYRMTFN KDFLIDLIGY RRFGHNEMDE PSATQPMLYD 490 500 510 520 530 540 AVRKHPTVKN IFAEKLIHKG IVDKETVGKI KDAVQKRLEE AYRKVPAKKE DMTHEIVLPE 550 560 570 580 590 600 PVSNGFPDVD TSVDFETLRK INQELVSWPE NFNVFDKLKR ILERRAKAFE DDRKVDWSLA 610 620 630 640 650 660 EAMAFASILK DGTPLRLTGQ DSERGTFAHR NLVLHDSKTG DEFIALHHLA DTKASFAVHN 670 680 690 700 710 720 SPLSEGSVLG FEYGYNVSSP ETMVIWEAQF GDFANAAQVY FDQFISAGRA KWGQKSGLVV 730 740 750 760 770 780 LLPHGYEGQG PEHSSGRTER FLQLAAENNW TVANLTSAAQ YFHILRRQAK MLLREEIRPL 790 800 810 820 830 840 IIMTPKSLLR NPNTVSEVQE LSNSSFKPVY EMSGLSHQYD KVTRLVLSSG KVSIDISDHF 850 860 870 880 890 900 NKMEGEKDWL HIARVEELYP FPAKHIKAIF SKLPNLEEIV WVQEEPQNMG AWNYIEPYLR 910 920 930 940 EVAPKDVKVR YIGRRRRSSP AEGDPTVHKK EQERIVSDSL TRKN

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41140.1. CP000002 Genomic DNA. Translation: AAU23783.1.
RefSeq	YP_079421.1. YP_091833.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3028258. 3100388.
GenomeReviews	Gene locus BL01452 in contig CP000002_GR. Gene locus BLi02260 in contig AE017333_GR.
KEGG	bld:BLi02260. bli:BL01452.
NMPDR	fig|279010.5.peg.30.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65IH4.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01452-MON.
Family and domain databases
HAMAP	MF_01169. [Tree]
InterPro	IPR011603. 2oxoglutarate_DHase_E1. IPR001017. DHase_E1. IPR005475. Transketo_Cen_R. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_BACLD

Accession

Primary (citable) accession number: Q65IH4
Secondary accession number(s): Q62TX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	October 25, 2004
Last modified:	November 4, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)