Q65IB0 (ILVD_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BLi02324, BL05225

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225369
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65IB0 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 1A9029F7205ABB0B	FASTA	558	59,461
	10 20 30 40 50 60 MTGLRSDMIT KGIDRAPHRS LLRAAGVKEE DFGKPFIAVC NSYIDIVPGH VHLQEFGKIV 70 80 90 100 110 120 KEAIREAGGV PFEFNTIGVD DGIAMGHIGM RYSLPSREII ADSVETVVSA HWFDGMVCIP 130 140 150 160 170 180 NCDKITPGMI MAAMRINIPT VFVSGGPMEA GRTSDGRKIS LSSVFEGVGA YQSGKIDEKG 190 200 210 220 230 240 LEELEQFGCP TCGSCSGMFT ANSMNCLSEA LGIAMPGNGT ILATSPDRRE FAKQSARQLM 250 260 270 280 290 300 ELIKSDIKPR DIVTEKAIDN AFALDMALGG STNTILHTLA IANEAGVDYS LERINEVAAR 310 320 330 340 350 360 VPHLSKLAPA SDVFIEDLHE AGGVSAVLNE LSKKEGALHL DTLTVTGKTL GENIAGREVK 370 380 390 400 410 420 DYEVIHPIDQ PFSEQGGLAV LFGNLAPDGA IIKTGGVQDG ITRHEGPAVV FDSQEEALDG 430 440 450 460 470 480 IINRKVKAGD VVIIRYEGPK GGPGMPEMLA PTSQIVGMGL GPKVALITDG RFSGASRGLS 490 500 510 520 530 540 IGHVSPEAAE GGPLAFVENG DHIVVDIEKR ILNIEISDEE WEKRKANWPG FEPKVKTGYL 550 ARYSKLVTSA NTGGIMKI

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU41204.1. CP000002 Genomic DNA. Translation: AAU23848.1.
RefSeq	YP_079486.1. NC_006270.3. YP_091897.1. NC_006322.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65IB0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000055494; EBBACP00000054025; EBBACG00000055485. EBBACT00000061770; EBBACP00000060203; EBBACG00000061761.
GeneID	3029287. 3099313.
GenomeReviews	Gene locus BLi02324 in contig AE017333_GR. Gene locus BL05225 in contig CP000002_GR.
KEGG	bld:BLi02324. bli:BL05225.
NMPDR	fig|279010.5.peg.175.
PATRIC	18950300. VBIBacLic203714_2352.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
GeneTree	EBGT00050000001430.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q65IB0.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI02324-MONOMER. BLIC279010:BL05225-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACLD

Accession

Primary (citable) accession number: Q65IB0
Secondary accession number(s): Q62TR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program