Q65IA6 (FTHS_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	BLi02328, BL01370

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.
Sequence caution	The sequence AAU23852.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000199331
Regions
	Nucleotide binding	68 – 75	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65IA6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 281A86AE4B83A19A	FASTA	559	59,961
	10 20 30 40 50 60 MKSHLSDIEI AQRTELKPIT EIARKLNIND EEIECFGCTK AKISLKIFER LKEKPDGQVI 70 80 90 100 110 120 LVTSINPTPA GEGKSTVTVG LSQALWQIGK KSIVAMREPS LGPTMGLKGG AAGGGYSQVL 130 140 150 160 170 180 PMEDINLHFT GDMHAITAAN NALAAFIDNH IHQGNELNID IRKIVWKRAL DLNDRALRET 190 200 210 220 230 240 VVGLGGKANG FPREDGFDIT VASEIMAVLC LARDLADLKK RLASMIVAYT VDGQPVTAGM 250 260 270 280 290 300 LGVQGALALL LKDAIKPNLV QTVEGTPALV HGGPFANIAH GANSLIATKT AAKLADYVVT 310 320 330 340 350 360 EAGFGADLGA EKFMHIKTRA GGFTPGAVVI VATVRALKMH GGTPLADLKQ KDLEALKRGI 370 380 390 400 410 420 ANLAKHIETI DAFGLPYVVA VNRFVQDTED ELEAVLGWCR DNGHPAALCN VWEEGGKGGT 430 440 450 460 470 480 DLAREVIHVM EQKDNRFSYL YELTDSIEDK LAKISRTVYG AEGVEFTEKA KKQLLELKKN 490 500 510 520 530 540 GLDGLPVCVA KTQYSLSDDP GKIGRPQDFS ITVRELKPSR GAGFIVALTG SILTMPGLPK 550 HPAALKMDVD ESGRAKGLF

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU41208.1. CP000002 Genomic DNA. Translation: AAU23852.1. Different initiation.
RefSeq	YP_079490.1. NC_006270.3. YP_091901.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65IA6.
SMR	Q65IA6. Positions 7-557.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65IA6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000056774; EBBACP00000055305; EBBACG00000056765. EBBACT00000060525; EBBACP00000058958; EBBACG00000060516.
GeneID	3028622. 3100294.
GenomeReviews	Gene locus BLi02328 in contig AE017333_GR. Gene locus BL01370 in contig CP000002_GR.
KEGG	bld:BLi02328. bli:BL01370.
NMPDR	fig|279010.5.peg.179.
PATRIC	18950308. VBIBacLic203714_2356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
GeneTree	EBGT00050000004414.
HOGENOM	HBG677721.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI02328-MONOMER. BLIC279010:BL01370-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_BACLD

Accession

Primary (citable) accession number: Q65IA6
Secondary accession number(s): Q62TQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program