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Q65IA6 (FTHS_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:BLi02328, BL01370
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Sequence caution

The sequence AAU23852.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199331

Regions

Nucleotide binding68 – 758ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65IA6 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 281A86AE4B83A19A

FASTA55959,961
        10         20         30         40         50         60 
MKSHLSDIEI AQRTELKPIT EIARKLNIND EEIECFGCTK AKISLKIFER LKEKPDGQVI 

        70         80         90        100        110        120 
LVTSINPTPA GEGKSTVTVG LSQALWQIGK KSIVAMREPS LGPTMGLKGG AAGGGYSQVL 

       130        140        150        160        170        180 
PMEDINLHFT GDMHAITAAN NALAAFIDNH IHQGNELNID IRKIVWKRAL DLNDRALRET 

       190        200        210        220        230        240 
VVGLGGKANG FPREDGFDIT VASEIMAVLC LARDLADLKK RLASMIVAYT VDGQPVTAGM 

       250        260        270        280        290        300 
LGVQGALALL LKDAIKPNLV QTVEGTPALV HGGPFANIAH GANSLIATKT AAKLADYVVT 

       310        320        330        340        350        360 
EAGFGADLGA EKFMHIKTRA GGFTPGAVVI VATVRALKMH GGTPLADLKQ KDLEALKRGI 

       370        380        390        400        410        420 
ANLAKHIETI DAFGLPYVVA VNRFVQDTED ELEAVLGWCR DNGHPAALCN VWEEGGKGGT 

       430        440        450        460        470        480 
DLAREVIHVM EQKDNRFSYL YELTDSIEDK LAKISRTVYG AEGVEFTEKA KKQLLELKKN 

       490        500        510        520        530        540 
GLDGLPVCVA KTQYSLSDDP GKIGRPQDFS ITVRELKPSR GAGFIVALTG SILTMPGLPK 

       550 
HPAALKMDVD ESGRAKGLF 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017333 Genomic DNA. Translation: AAU41208.1.
CP000002 Genomic DNA. Translation: AAU23852.1. Different initiation.
RefSeqYP_006713689.1. NC_006322.1.
YP_079490.1. NC_006270.3.

3D structure databases

ProteinModelPortalQ65IA6.
SMRQ65IA6. Positions 7-557.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL01370.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU23852; AAU23852; BL01370.
AAU41208; AAU41208; BLi02328.
GeneID3028622.
3100294.
KEGGbld:BLi02328.
bli:BL01370.
PATRIC18950308. VBIBacLic203714_2356.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-2314-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_BACLD
AccessionPrimary (citable) accession number: Q65IA6
Secondary accession number(s): Q62TQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 25, 2004
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways