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Protein

Processive diacylglycerol beta-glucosyltransferase

Gene

ugtP

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA).UniRule annotation

Catalytic activityi

UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.UniRule annotation

Pathwayi: diglucosyl-diacylglycerol biosynthesis

This protein is involved in the pathway diglucosyl-diacylglycerol biosynthesis, which is part of Glycolipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway diglucosyl-diacylglycerol biosynthesis and in Glycolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-2316-MONOMER.
UniPathwayiUPA00894.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Processive diacylglycerol beta-glucosyltransferaseUniRule annotation (EC:2.4.1.315)
Alternative name(s):
Beta-diglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-DGSUniRule annotation
Short name:
DGlcDAG synthaseUniRule annotation
Short name:
Glc2-DAG synthaseUniRule annotation
Beta-gentiobiosyldiacylglycerol synthaseUniRule annotation
Beta-monoglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-MGSUniRule annotation
Short name:
MGlcDAG synthaseUniRule annotation
Beta-triglucosyldiacylglycerol synthaseUniRule annotation
Short name:
TGlcDAG synthaseUniRule annotation
Diglucosyl diacylglycerol synthase (1,6-linking)
Glucosyl-beta-1,6-glucosyldiacylglycerol synthaseUniRule annotation
UDP glucosyltransferaseUniRule annotation
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferaseUniRule annotation
Gene namesi
Name:ugtPUniRule annotation
Ordered Locus Names:BLi02330, BL01366
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Processive diacylglycerol beta-glucosyltransferasePRO_0000308452Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279010.BLi02330.

Structurei

3D structure databases

ProteinModelPortaliQ65IA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. UgtP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108QBV. Bacteria.
COG0707. LUCA.
HOGENOMiHOG000221565.
KOiK03429.
OMAiYKAFYYS.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr. 1 hit.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR001296. Glyco_trans_1.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65IA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTNKNILIL TANYGNGHVQ VAKTLYQECE RLGFKNVTVS NLYQESNPIV
60 70 80 90 100
SEITQYLYLK SFSIGKQFYR LFYYGVDKIY NKRKFNIYFK MGNKRLDQLV
110 120 130 140 150
KKHQPDIIIN TFPMIVVPEY RRRMGKVIPT FNVMTDFCLH KIWVHEHIDK
160 170 180 190 200
YYVATDYVKE KLLEIGTHPS NVKITGIPIR RQFEEEMDKD KIYEKYQLSP
210 220 230 240 250
DKKILLIMAG AHGVLKNVKE LCESLVTKED VQVVVVCGKN TMLKSSLEDI
260 270 280 290 300
EALYPNKLRT LGYIERIDEL FRVADCMITK PGGITLTEAT AIGVPVILYK
310 320 330 340 350
PVPGQEKENA LFFEDRGAAI VVNRHEEILE SVSSLLADEK KLNEMKKNIK
360 370 380
SLHLSNSSEV ILTDIIEQSE IIMNKKQTVR ALS
Length:383
Mass (Da):44,168
Last modified:October 25, 2004 - v1
Checksum:i24098768FDF29363
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU23854.1.
AE017333 Genomic DNA. Translation: AAU41210.1.
RefSeqiWP_003182832.1. NC_006322.1.

Genome annotation databases

EnsemblBacteriaiAAU23854; AAU23854; BL01366.
AAU41210; AAU41210; BLi02330.
GeneIDi3027840.
KEGGibld:BLi02330.
bli:BL01366.
PATRICi18950312. VBIBacLic203714_2358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU23854.1.
AE017333 Genomic DNA. Translation: AAU41210.1.
RefSeqiWP_003182832.1. NC_006322.1.

3D structure databases

ProteinModelPortaliQ65IA4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi02330.

Protein family/group databases

CAZyiGT28. Glycosyltransferase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU23854; AAU23854; BL01366.
AAU41210; AAU41210; BLi02330.
GeneIDi3027840.
KEGGibld:BLi02330.
bli:BL01366.
PATRICi18950312. VBIBacLic203714_2358.

Phylogenomic databases

eggNOGiENOG4108QBV. Bacteria.
COG0707. LUCA.
HOGENOMiHOG000221565.
KOiK03429.
OMAiYKAFYYS.

Enzyme and pathway databases

UniPathwayiUPA00894.
BioCyciBLIC279010:GJ2P-2316-MONOMER.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr. 1 hit.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR001296. Glyco_trans_1.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUGTP_BACLD
AccessioniPrimary (citable) accession number: Q65IA4
Secondary accession number(s): Q62TQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 25, 2004
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.