Reviewed, UniProtKB/Swiss-Prot Q65I39 (AROA_BACLD)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BLi02395, BL02766

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000325332

Sequences

	Sequence		Length	Mass (Da)
	Q65I39-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: FB722BAFCAF57F53	FASTA	428	45,522
	10 20 30 40 50 60 MKRGKISSLK GELHIPGDKS ISHRSVMFGA MAEGKTVIKN FLPGADCLST IACFRKMGVE 70 80 90 100 110 120 IEQNGSDVTV RGKGLDQLAE PAELLDVGNS GTTIRLMLGI LAGRPFHSTV AGDESIAKRP 130 140 150 160 170 180 MKRVTEPLRK MGAKIDGRAG GEYTPLSVRG GHLKAIDFQS PVASAQIKSA VLLAGLQAEG 190 200 210 220 230 240 TTTVTEPHKS RDHTERMLSM FGVSLREDET SVSIEGGQQL KAAEVFVPGD ISSAAFFLAA 250 260 270 280 290 300 ASLVPGSEVV LRNVGLNPTR TGIIDVLKEM GADLEIEEKD TGNTEPYGDL RIKTSSLKAA 310 320 330 340 350 360 EISGDLIPRL IDEIPIIALL ATQAEGTTVI KDAAELKVKE TNRIDTVASE LKKIGANIEP 370 380 390 400 410 420 TEDGMKIHGK TPLTGGAKVS SHGDHRIGMM LGIAACICEQ PIDILQPEAV SVSYPSFFEH IEKLAEKA

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000002 Genomic DNA. Translation: AAU23920.3. AE017333 Genomic DNA. Translation: AAU41275.1.
RefSeq	YP_079558.3. YP_091968.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65I39.
Genome annotation databases
GeneID	3029401. 3101463.
GenomeReviews	Gene locus BLi02395 in contig AE017333_GR. Gene locus BL02766 in contig CP000002_GR.
KEGG	bld:BLi02395. bli:BL02766.
NMPDR	fig|279010.5.peg.245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65I39.
Family and domain databases
HAMAP	MF_00210. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BACLD

Accession

Primary (citable) accession number: Q65I39
Secondary accession number(s): Q62TI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 25, 2004
Last modified:	September 22, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)