Reviewed, UniProtKB/Swiss-Prot Q65HN9 (NAMA_BACLD)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BLi02551, BL00797

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	NADPH dehydrogenase HAMAP MF_01614		PRO_0000216118
Regions
	Nucleotide binding	24 – 28	5	FMN By similarity
Sites
	Binding site	29	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	216	1	FMN By similarity
	Binding site	309	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65HN9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: A6AE937304B34654	FASTA	339	37,405
	10 20 30 40 50 60 MSNRKLFTPW SLKGVTLKNR IVMSPMCMYS SHEKDGKVQP FHMTHYISRA VGQVGLIMVE 70 80 90 100 110 120 ATAVTPQGRI SDQDLGIWDD AHIDGLAALT SQIKTYGSKT AIQLAHAGRK AEVEGTIYGP 130 140 150 160 170 180 SAIPFDENSR TPVEMTKEDI KETVQAFKKG AERAKAAGFD IIEIHGAHGY LINEFLSPLS 190 200 210 220 230 240 NKREDEYGGS PENRYRLLRE VIDAVKEVWA GPLFVRVSAS DYKTKGLDVA DYVGFAKWMK 250 260 270 280 290 300 EQGVDLIDVS SGAVVPADIN VFPGYQVGFA DTIRAQAEIQ TGAVGLITSG LQAEEILQNG 310 320 330 RADLIFVARE LLRDPYWPKT AAKQLNTKIE GPVQYDRAW

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41425.1. CP000002 Genomic DNA. Translation: AAU24067.1.
RefSeq	YP_079705.1. YP_092118.1.
3D structure databases
SMR	Q65HN9. Positions 4-339.
ModBase	Search...
Genome annotation databases
GeneID	3028377. 3100096.
GenomeReviews	Gene locus BLi02551 in contig AE017333_GR. Gene locus BL00797 in contig CP000002_GR.
KEGG	bld:BLi02551. bli:BL00797.
NMPDR	fig|279010.5.peg.396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65HN9.
OMA	Q65HN9. WVEDGWN.
Enzyme and pathway databases
BioCyc	BLIC279010:BL00797-MON.
Family and domain databases
HAMAP	MF_01614. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACLD

Accession

Primary (citable) accession number: Q65HN9
Secondary accession number(s): Q62T42

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)