Q65HJ2 (DXS_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BLi02598, BL01523

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256378

Sequences

	Sequence		Length	Mass (Da)
	Q65HJ2 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: B83574356855009A	FASTA	633	69,788
	10 20 30 40 50 60 MDLLSIKDPA FLKNLSNEEL ESLSAEIRRF LIETLSESGG HIGPNLGVVE LTIALHKEFD 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKLL TGRGKDFATL RQHKGLCGFP KRNESEHDVW ETGHSSTSLS 130 140 150 160 170 180 GAMGMAAARD LKGTNEYIIP IIGDGALTGG MALEALNHIG HEQKDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGAIHSMLGR LRTAGKYQWV KDELEYLFKR IPAVGGKLAA TAERIKDSLK YLLVSGMFFE 250 260 270 280 290 300 ELGFTYLGPV DGHSYDELFE NLQYAKKTKG PVLLHVITKK GKGYKPAETD KTGTWHGTGP 310 320 330 340 350 360 YKIDTGDFVK PKAAAPSWSS LVSETVRKLA REDERIVAIT PAMPVGSKLE GFASEFPERM 370 380 390 400 410 420 FDVGIAEQHA ATMAAGLATQ NMKPFLAIYS TFLQRAYDQV VHDICRQNLN VFIGIDRAGL 430 440 450 460 470 480 VGADGETHQG VFDIAFLRHI PNLVLMMPKD ENEGQHMVNT AVKYDDGPIA MRFPRGNGLG 490 500 510 520 530 540 VKMDKELKTI PIGTWEVLRP GTDAVILTFG TTIPMALAAA EELQKEGRSV RVVNARFIKP 550 560 570 580 590 600 LDENMLKEIL NEGLPILTIE EAVLQGGFGS SILEFAHEHQ SYSPIIDRMG IPDQFIEHGS 610 620 630 VAKLLEEIGM TKEDVIRRIR LLTPVKTHKG IGS

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU41472.1. CP000002 Genomic DNA. Translation: AAU24113.2.
RefSeq	YP_079751.2. NC_006270.3. YP_092165.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65HJ2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65HJ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000055534; EBBACP00000054065; EBBACG00000055525. EBBACT00000058504; EBBACP00000056937; EBBACG00000058495.
GeneID	3029280. 3099865.
GenomeReviews	Gene locus BLi02598 in contig AE017333_GR. Gene locus BL01523 in contig CP000002_GR.
KEGG	bld:BLi02598. bli:BL01523.
NMPDR	fig|279010.5.peg.441.
PATRIC	18950870. VBIBacLic203714_2637.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
GeneTree	EBGT00070000031844.
HOGENOM	HBG571647.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI02598-MONOMER. BLIC279010:BL01523-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACLD

Accession

Primary (citable) accession number: Q65HJ2
Secondary accession number(s): Q62SZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program