Q65HF9 (GCST_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BLi02631, BL01562

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Aminomethyltransferase HAMAP MF_00259		PRO_1000059080

Sequences

	Sequence		Length	Mass (Da)
	Q65HF9 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: E59B2CEFD915EA2D	FASTA	364	40,000
	10 20 30 40 50 60 MLKRTPLFDL YKEYGGKTID FGGWELPVQF SSIKEEHEAV RTKAGLFDVS HMGEVEITGT 70 80 90 100 110 120 DSLPFLQKLL TNDVSTLKEG GAQYTAMCYE DGGTIDDLLV YKKAANVYML VINAANIDKD 130 140 150 160 170 180 VDWMNKHIKG DVSVRNVSDE IALLALQGPK AEAILKQVAD HDLAELKPFM FRDDAAVGSV 190 200 210 220 230 240 QALVSRTGYT GEDGFEIYCR NEDAACIWKL LLETGKDSGL VPCGLGARDT LRFEAKLPLY 250 260 270 280 290 300 GQELSKDITP IEAGIGFAVK TNKASDFIGK AVLASQKEHG ADRKLVGLEM IDKGIPRHGY 310 320 330 340 350 360 AVYYQGEQAG EVTTGTQSPT LKKNVGLALL KKEACALDTV VEVEIRNKRL KAKIVKTPFY KRQP

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU24146.1. AE017333 Genomic DNA. Translation: AAU41505.1.
RefSeq	YP_079784.1. NC_006270.3. YP_092198.1. NC_006322.1.
3D structure databases
HSSP	HSSP built from PDB template 1YX2 based on UniProtKB P54378.
ProteinModelPortal	Q65HF9.
SMR	Q65HF9. Positions 1-362.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65HF9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000057364; EBBACP00000055895; EBBACG00000057355. EBBACT00000059689; EBBACP00000058122; EBBACG00000059680.
GeneID	3028919. 3097439.
GenomeReviews	Gene locus BLi02631 in contig AE017333_GR. Gene locus BL01562 in contig CP000002_GR.
KEGG	bld:BLi02631. bli:BL01562.
NMPDR	fig|279010.5.peg.476.
PATRIC	18950944. VBIBacLic203714_2674.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
GeneTree	EBGT00050000001932.
HOGENOM	HBG299834.
OMA	VINASNK.
PhylomeDB	Q65HF9.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI02631-MONOMER. BLIC279010:BL01562-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BACLD

Accession

Primary (citable) accession number: Q65HF9
Secondary accession number(s): Q62SW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program