Reviewed, UniProtKB/Swiss-Prot Q65H34 (AROE_BACLD)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BLi02759, BL02079

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021263
Regions
	Nucleotide binding	127 – 131	5	NADP By similarity
Sites
	Active site	66	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65H34-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 57F83D4D8F67A14E	FASTA	278	29,909
	10 20 30 40 50 60 MKPIYGLIGN PVAHSMSPDI HNAALKDLSL EGHYHAFRVE NEDLEDAVKG MRALGIQGFN 70 80 90 100 110 120 VTVPHKVSIM KHLDRIDESA EALGAVNTVR REKEGLVGYN TDGAGFLKSL KPSLDRPLSE 130 140 150 160 170 180 LSILLIGAGG AARAIFTTLA AETPKRLDVA NRTPEKALAF TQRFDGEARA LSLQEAEADL 190 200 210 220 230 240 SAYDIVIQTT SVGMHPNVEA APLSLANAKE TCLVCDIIYN PLKTALLHEA EAKGLKTLDG 250 260 270 VGMFIGQAAL AFELWTGHEP NMEKMKSIVL QQLGGKSC

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU24269.1. AE017333 Genomic DNA. Translation: AAU41630.1.
RefSeq	YP_079907.1. YP_092323.1.
3D structure databases
HSSP	HSSP built from PDB template 1NVT based on UniProtKB Q58484.
SMR	Q65H34. Positions 1-273.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65H34.
Genome annotation databases
GeneID	3028801. 3097844.
GenomeReviews	Gene locus BLi02759 in contig AE017333_GR. Gene locus BL02079 in contig CP000002_GR.
KEGG	bld:BLi02759. bli:BL02079.
NMPDR	fig|279010.5.peg.3030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	MLINATT.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI02759-MONOMER. BLIC279010:BL02079-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BACLD

Accession

Primary (citable) accession number: Q65H34
Secondary accession number(s): Q62SJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)