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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62ShikimateUniRule annotation1
Active sitei66Proton acceptorUniRule annotation1
Binding sitei78NADPUniRule annotation1
Binding sitei87ShikimateUniRule annotation1
Binding sitei102ShikimateUniRule annotation1
Binding sitei217NADP; via carbonyl oxygenUniRule annotation1
Binding sitei219ShikimateUniRule annotation1
Binding sitei240NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 131NADPUniRule annotation5
Nucleotide bindingi151 – 156NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:BLi02759, BL02079
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000212631 – 278Shikimate dehydrogenase (NADP(+))Add BLAST278

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi02759.

Structurei

3D structure databases

ProteinModelPortaliQ65H34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiQPYGNIQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65H34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPIYGLIGN PVAHSMSPDI HNAALKDLSL EGHYHAFRVE NEDLEDAVKG
60 70 80 90 100
MRALGIQGFN VTVPHKVSIM KHLDRIDESA EALGAVNTVR REKEGLVGYN
110 120 130 140 150
TDGAGFLKSL KPSLDRPLSE LSILLIGAGG AARAIFTTLA AETPKRLDVA
160 170 180 190 200
NRTPEKALAF TQRFDGEARA LSLQEAEADL SAYDIVIQTT SVGMHPNVEA
210 220 230 240 250
APLSLANAKE TCLVCDIIYN PLKTALLHEA EAKGLKTLDG VGMFIGQAAL
260 270
AFELWTGHEP NMEKMKSIVL QQLGGKSC
Length:278
Mass (Da):29,909
Last modified:October 25, 2004 - v1
Checksum:i57F83D4D8F67A14E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU24269.1.
AE017333 Genomic DNA. Translation: AAU41630.1.
RefSeqiWP_003183706.1. NC_006322.1.

Genome annotation databases

EnsemblBacteriaiAAU24269; AAU24269; BL02079.
AAU41630; AAU41630; BLi02759.
GeneIDi3028801.
KEGGibld:BLi02759.
bli:BL02079.
PATRICi18951206. VBIBacLic203714_2804.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU24269.1.
AE017333 Genomic DNA. Translation: AAU41630.1.
RefSeqiWP_003183706.1. NC_006322.1.

3D structure databases

ProteinModelPortaliQ65H34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi02759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU24269; AAU24269; BL02079.
AAU41630; AAU41630; BLi02759.
GeneIDi3028801.
KEGGibld:BLi02759.
bli:BL02079.
PATRICi18951206. VBIBacLic203714_2804.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiQPYGNIQ.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_BACLD
AccessioniPrimary (citable) accession number: Q65H34
Secondary accession number(s): Q62SJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.