Reviewed, UniProtKB/Swiss-Prot Q65GR4 (SYD_BACLD)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BLi02881, BL05288

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00044
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110827

Sequences

	Sequence		Length	Mass (Da)
	Q65GR4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: EBD733AB72A2EAEC	FASTA	592	66,378
	10 20 30 40 50 60 MFGRTYYCGE ITEKAIGETV VLKGWVQKRR DLGGLIFIDL RDRTGIVQVV FNPDVSKDAL 70 80 90 100 110 120 ETAESIRSEY VLDITGKVVA REEATVNPNL KTGRIEIQAE SVDVLSAAKT PPFAISDQAA 130 140 150 160 170 180 EVSEDIRLKH RYLDLRRPEM FNSLKMRHNV TKAVRRFLDD NGFLDIETPV LTKSTPEGAR 190 200 210 220 230 240 DYLVPSRVHE GEFYALPQSP QIFKQLLMVS GFDRYYQIAR CFRDEDLRAD RQPEFTQIDI 250 260 270 280 290 300 EMSFMSQEDI MKLSEEMMAH VMRETHGIEI SLPLPRMSYE DAMNRYGSDK PDTRFGMLLT 310 320 330 340 350 360 DVSEAVKDSD FKVFASAVAN GGAVKAINVK GAAANYSRKD IDALGEFAAN YGAKGLAWLK 370 380 390 400 410 420 TEADGLKGPI AKFFAGEKQE ALVQALDAAD GDLLLFVADK LEVANDALGA LRLKLGKELN 430 440 450 460 470 480 LIDESLFNFL WVVDWPLLEY DAEEGRYYAA HHPFTMPVRE DLKLIETNPQ DMKAQAYDLV 490 500 510 520 530 540 LNGYELGGGS IRIFEKDVQE KMFGLLGFSE EEAKEQFGFL LEAFDYGAPP HGGIALGLDR 550 560 570 580 590 LVMLLSGRTN LRDTIAFPKT ASASCLMTEA PSEVDNAQLD ELHLEIKRKI RQ

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41750.1. CP000002 Genomic DNA. Translation: AAU24387.1.
RefSeq	YP_080025.1. YP_092443.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65GR4.
Genome annotation databases
GeneID	3029050. 3098196.
GenomeReviews	Gene locus BLi02881 in contig AE017333_GR. Gene locus BL05288 in contig CP000002_GR.
KEGG	bld:BLi02881. bli:BL05288.
NMPDR	fig|279010.5.peg.3209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65GR4.
OMA	VDRRRDH.
Enzyme and pathway databases
BioCyc	BLIC279010:BL05288-MON.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACLD

Accession

Primary (citable) accession number: Q65GR4
Secondary accession number(s): Q62S73

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)