Reviewed, UniProtKB/Swiss-Prot Q65GP9 (TGT_BACLD)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	BLi02897, BL01140

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135450
Sites
	Active site	96	1	Nucleophile By similarity
	Metal binding	308	1	Zinc By similarity
	Metal binding	310	1	Zinc By similarity
	Metal binding	313	1	Zinc By similarity
	Metal binding	339	1	Zinc By similarity
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65GP9-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 235D1C594D363823	FASTA	381	43,615
	10 20 30 40 50 60 MSQQPIRYEF IKACKQTGAR LGRVHTPHGS FETPVFMPVG TLATVKTMSP EELKEMGADI 70 80 90 100 110 120 ILSNTYHLWL RPGHDIVKEA GGLHQFMNWN RAILTDSGGF QVFSLSEFRK IEEEGVHFRN 130 140 150 160 170 180 HLNGDKLFLS PEKAMEIQNA LGSDIMMAFD ECPPYPAEYD YMKKSVERTS RWAERCLKAH 190 200 210 220 230 240 NRPDEQGLFG IVQGGEYENL RKQSAKDLIS LDFPGYAIGG LSVGEPKDVM NRVLEFTTPL 250 260 270 280 290 300 LPADKPRYLM GVGSPDSLID GAIRGVDMFD CVLPTRIARN GTLMTSEGRL VVKNAKYERD 310 320 330 340 350 360 FRPIDEQCDC YTCRNYSRAY IRHLIRCNET FGIRLTSYHN LYFLLKLMGQ VRDAIREDRL 370 380 GDFREEFFER YGFNKPNAKS F

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41765.1. CP000002 Genomic DNA. Translation: AAU24402.1.
RefSeq	YP_080040.1. YP_092458.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3028240. 3098221.
GenomeReviews	Gene locus BLi02897 in contig AE017333_GR. Gene locus BL01140 in contig CP000002_GR.
KEGG	bld:BLi02897. bli:BL01140.
NMPDR	fig|279010.5.peg.3224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65GP9.
OMA	Q65GP9. MAFDQCP.
Enzyme and pathway databases
BioCyc	BLIC279010:BL01140-MON.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_BACLD

Accession

Primary (citable) accession number: Q65GP9
Secondary accession number(s): Q62S58

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)