Reviewed, UniProtKB/Swiss-Prot Q65GI9 (LEU3_BACLD)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	BLi02957, BL00612

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	3-isopropylmalate dehydrogenase HAMAP MF_01033		PRO_0000083640
Regions
	Nucleotide binding	76 – 89	14	NAD By similarity
	Nucleotide binding	282 – 294	13	NAD By similarity
Sites
	Metal binding	224	1	Magnesium or manganese By similarity
	Metal binding	248	1	Magnesium or manganese By similarity
	Metal binding	252	1	Magnesium or manganese By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	224	1	Substrate By similarity
	Site	141	1	Important for catalysis By similarity
	Site	192	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65GI9-1 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 9F23133E6AECED1C	FASTA	370	39,850
	10 20 30 40 50 60 MKKRIALLPG DGIGPEVLDA AVDVLKSVAE HYQHEFEFEY GLIGGAAIDE AGAPLPEETV 70 80 90 100 110 120 AACKAADAIL LGAVGGPKWD QNPSELRPEK GLLAIRKQLD LFANLRPVKV FESLAGASPL 130 140 150 160 170 180 KTEYIEGVDF VIVRELTGGL YFGKPSEQYV NQNGEEEAVD TLFYKKSEME RVIREAFQMA 190 200 210 220 230 240 QSRKGKVTSV DKANVLESSK LWRKTAEEVA KEFPDVKLEH MLVDNAAMQL IYAPGQFDII 250 260 270 280 290 300 VTENMFGDIL SDEASMLTGS LGMLPSASLS SSGLHLYEPV HGSAPDIAGQ NIANPLAAIL 310 320 330 340 350 360 SAAMMLRTSF GLEAEAQAVE HAVDQVLRAG KRTKDLAKGS EHCTTQSITG EVKAALADDN 370 AISNIMTAYV

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41825.1. Different initiation. CP000002 Genomic DNA. Translation: AAU24463.1.
RefSeq	YP_080101.1. YP_092518.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3028335. 3098436.
GenomeReviews	Gene locus BLi02957 in contig AE017333_GR. Gene locus BL00612 in contig CP000002_GR.
KEGG	bld:BLi02957. bli:BL00612.
NMPDR	fig|279010.5.peg.3307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65GI9.
OMA	Q65GI9. EAFDTMR.
Enzyme and pathway databases
BioCyc	BLIC279010:BL00612-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BACLD

Accession

Primary (citable) accession number: Q65GI9
Secondary accession number(s): Q62RZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	January 10, 2006
Last modified:	June 16, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)