Reviewed, UniProtKB/Swiss-Prot Q65GI7 (ILVC_BACLD)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BLi02959, BL00610

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Ketol-acid reductoisomerase		PRO_0000226158
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65GI7-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 2FA57CDEB5709B10	FASTA	342	37,469
	10 20 30 40 50 60 MVKVYYNGDI KENVLAGKKV AVIGYGSQGH AHALNLKESG VDVIVGVRKG KSFAKAEEDG 70 80 90 100 110 120 HQVFTVREAA EQADIVMVLL PDEQQQKVYE AEIKDGLQAG NSLVFAHGFN VHFHQIVPPA 130 140 150 160 170 180 DVDVFLVAPK GPGHLVRRTF EQGAGVPALF AIYQDVTGEA KDKALAYAKA IGGARAGVLE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GLTSLVKAGF ETLTEAGYQP ELAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 EGLAGMRYSI SDTAQWGDFV SGPRVVDEKV KESMKEVLKD IQNGTFAKEW IVENQVNRPR 310 320 330 340 FNAINASENE HQIEVVGRKL REMMPFVKQG KKKEAVVAGA KN

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017333 Genomic DNA. Translation: AAU41827.1. CP000002 Genomic DNA. Translation: AAU24465.1.
RefSeq	YP_080103.1. YP_092520.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3028371. 3098442.
GenomeReviews	Gene locus BL00610 in contig CP000002_GR. Gene locus BLi02959 in contig AE017333_GR.
KEGG	bld:BLi02959. bli:BL00610.
NMPDR	fig|279010.5.peg.3309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65GI7.
Enzyme and pathway databases
BioCyc	BLIC279010:BL00610-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BACLD

Accession

Primary (citable) accession number: Q65GI7
Secondary accession number(s): Q62RZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 25, 2004
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)