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Q65GG2 (RNPH_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease PH

Short name=RNase PH
EC=2.7.7.56
Alternative name(s):
tRNA nucleotidyltransferase
Gene names
Name:rph
Ordered Locus Names:BLi02985, BL00312
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564

Catalytic activity

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564

Sequence similarities

Belongs to the RNase PH family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiontRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA nucleotidyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

tRNA-specific ribonuclease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Ribonuclease PH HAMAP-Rule MF_00564
PRO_1000024777

Sequences

Sequence LengthMass (Da)Tools
Q65GG2 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 86BFDAD5A433B890

FASTA24626,845
        10         20         30         40         50         60 
MRYDGRKNNE LRPVTMDLDF ITHPEGSVLI TVGGTKVICN ASVEDRVPPF LRGEGKGWIT 

        70         80         90        100        110        120 
AEYSMLPRAT NQRTIRESSK GKISGRTMEI QRLIGRALRA VVDLEKLGER TIWIDCDVIQ 

       130        140        150        160        170        180 
ADGGTRTASI TGAFTAMALA VGRLVEKGAL KEMPITDFLA ATSVGIDKEK GLILDLNYQE 

       190        200        210        220        230        240 
DSAAEVDMNV VMTGEGRFVE LQGTGEEATF SRSELDGLLE LAEKGIAELL EKQKEVLGDV 


ALSIQQ 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000002 Genomic DNA. Translation: AAU24491.2.
AE017333 Genomic DNA. Translation: AAU41852.1.
RefSeqYP_006714322.1. NC_006322.1.
YP_080129.2. NC_006270.3.

3D structure databases

ProteinModelPortalQ65GG2.
SMRQ65GG2. Positions 1-239.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL00312.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU24491; AAU24491; BL00312.
AAU41852; AAU41852; BLi02985.
GeneID3028501.
3098495.
KEGGbld:BLi02985.
bli:BL00312.
PATRIC18951694. VBIBacLic203714_3047.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0689.
HOGENOMHOG000229516.
KOK00989.
OrthoDBEOG6CZQQP.
ProtClustDBPRK00173.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-2960-MONOMER.

Family and domain databases

Gene3D3.30.230.70. 1 hit.
HAMAPMF_00564. RNase_PH.
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsTIGR01966. RNasePH. 1 hit.
PROSITEPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNPH_BACLD
AccessionPrimary (citable) accession number: Q65GG2
Secondary accession number(s): Q62RW9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families