Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease PH

Gene

rph

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86Phosphate (substrate) bindingUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:BLi02985, BL00312
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000247771 – 246Ribonuclease PHAdd BLAST246

Proteomic databases

PRIDEiQ65GG2

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi02985

Structurei

3D structure databases

ProteinModelPortaliQ65GG2
SMRiQ65GG2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 126Phosphate (substrate) bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ED0 Bacteria
COG0689 LUCA
HOGENOMiHOG000229516
KOiK00989
OMAiKGKGQGW

Family and domain databases

CDDicd11362 RNase_PH_bact, 1 hit
Gene3Di3.30.230.70, 1 hit
HAMAPiMF_00564 RNase_PH, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR002381 RNase_PH_bac-type
IPR018336 RNase_PH_CS
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55666 SSF55666, 1 hit
TIGRFAMsiTIGR01966 RNasePH, 1 hit
PROSITEiView protein in PROSITE
PS01277 RIBONUCLEASE_PH, 1 hit

Sequencei

Sequence statusi: Complete.

Q65GG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYDGRKNNE LRPVTMDLDF ITHPEGSVLI TVGGTKVICN ASVEDRVPPF
60 70 80 90 100
LRGEGKGWIT AEYSMLPRAT NQRTIRESSK GKISGRTMEI QRLIGRALRA
110 120 130 140 150
VVDLEKLGER TIWIDCDVIQ ADGGTRTASI TGAFTAMALA VGRLVEKGAL
160 170 180 190 200
KEMPITDFLA ATSVGIDKEK GLILDLNYQE DSAAEVDMNV VMTGEGRFVE
210 220 230 240
LQGTGEEATF SRSELDGLLE LAEKGIAELL EKQKEVLGDV ALSIQQ
Length:246
Mass (Da):26,845
Last modified:October 25, 2004 - v1
Checksum:i86BFDAD5A433B890
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA Translation: AAU24491.2
AE017333 Genomic DNA Translation: AAU41852.1
RefSeqiWP_009329330.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU24491; AAU24491; BL00312
AAU41852; AAU41852; BLi02985
GeneIDi3028501
KEGGibld:BLi02985
bli:BL00312

Similar proteinsi

Entry informationi

Entry nameiRNPH_BACLD
AccessioniPrimary (citable) accession number: Q65GG2
Secondary accession number(s): Q62RW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2004
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health