Q65GD2 (SYFA_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BLi03016, BL00340

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000231962
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65GD2 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 91FF113F0C3FD99E	FASTA	344	38,798
	10 20 30 40 50 60 MQEELKRLEK EAVEKVEAAG SLKEVNDVRV AYLGKKGPIT EVLRGMGKLS AEERPKMGAL 70 80 90 100 110 120 ANEVREKIAA AIAEKNARLE EEEVKRKLKE QTIDVTLPGS PVKTGARHPL TIVIEEIEDL 130 140 150 160 170 180 FISMGYSVEE GPEVETDYYN FEALNLPKEH PARDMQDSFY ITEDTLMRTQ TSPVQTRTME 190 200 210 220 230 240 KHKGKGPVKI ICPGKVYRRD NDDATHSHQF MQIEGLCVDR DISMSDLKGT LETVAKKMFG 250 260 270 280 290 300 EEREIRLRPS FFPFTEPSVE VDVSCFKCGG KGCSVCKQTG WIEILGAGMV HPNVLEMAGF 310 320 330 340 DSKQYQGFAF GMGVERIAML KYGIDDIRHF YTNDVRFLSQ FKQA

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU24524.1. AE017333 Genomic DNA. Translation: AAU41882.1.
RefSeq	YP_080162.1. NC_006270.3. YP_092575.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65GD2.
SMR	Q65GD2. Positions 82-342.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65GD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000054761; EBBACP00000053292; EBBACG00000054752. EBBACT00000059050; EBBACP00000057483; EBBACG00000059041.
GeneID	3028881. 3098552.
GenomeReviews	Gene locus BLi03016 in contig AE017333_GR. Gene locus BL00340 in contig CP000002_GR.
KEGG	bld:BLi03016. bli:BL00340.
NMPDR	fig|279010.5.peg.3364.
PATRIC	18951758. VBIBacLic203714_3079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000002461.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q65GD2.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI03016-MONOMER. BLIC279010:BL00340-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BACLD

Accession

Primary (citable) accession number: Q65GD2
Secondary accession number(s): Q62RT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program