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Q65G67 (ASSY_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Argininosuccinate synthase
EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:BLi03084, BL00417
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005

Subunit structure

Homotetramer By similarity. HAMAP MF_00005

Subcellular location

Cytoplasm By similarity HAMAP MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

argininosuccinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Argininosuccinate synthase HAMAP MF_00005
PRO_0000148570

Regions

Nucleotide binding10 – 189ATP By similarity

Sites

Binding site871Citrulline By similarity
Binding site1171ATP; via amide nitrogen By similarity
Binding site1191Aspartate By similarity
Binding site1231Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1241Aspartate By similarity
Binding site1271Citrulline By similarity
Binding site1751Citrulline By similarity
Binding site1841Citrulline By similarity
Binding site2601Citrulline By similarity
Binding site2721Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65G67 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 7EE1AB856384E051

FASTA40344,687
        10         20         30         40         50         60 
MTQKKKVVLA YSGGLDTSVA IKWLQEQGYD VVACCLDVGE GKDLAFVQQK ALQVGASNSY 

        70         80         90        100        110        120 
VIDAKEEFAK EFALTALQAH TLYEGKYPLV SALSRPLIAK KLVEVAEKEN AQAVAHGCTG 

       130        140        150        160        170        180 
KGNDQVRFEV SIKSLNPDLE VLAPVREWKW SRDEEIAYAE KHGIPIPINL DSPYSIDQNL 

       190        200        210        220        230        240 
WGRSNECGVL EDPWAAPPEG AYDLTKPLEK TPDTPDVIEI AFEEGVPVSI DGTHYPLSDL 

       250        260        270        280        290        300 
ILKLNELAGA HGVGRIDHVE NRLVGIKSRE VYECPGAMTL IKAHKELEDL TLVKEVAHFK 

       310        320        330        340        350        360 
PIIEQKMAEV IYNGLWFSPL KDALSGFLKE TQKHVTGVVR VKLFKGHAIV EGRKSEYSLY 

       370        380        390        400 
DEKLATYTKD DAFDHHAAVG FIELWGLPTK VNSIVQKKEQ IKA

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017333 Genomic DNA. Translation: AAU41947.1.
CP000002 Genomic DNA. Translation: AAU24589.1.
RefSeqYP_080227.1. NC_006270.3.
YP_092640.1. NC_006322.1.

3D structure databases

ProteinModelPortalQ65G67.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ65G67.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000057257; EBBACP00000055788; EBBACG00000057248.
EBBACT00000061828; EBBACP00000060261; EBBACG00000061819.
GeneID3028521.
3098708.
GenomeReviewsGene locus BLi03084 in contig AE017333_GR.
Gene locus BL00417 in contig CP000002_GR.
KEGGbld:BLi03084.
bli:BL00417.
NMPDRfig|279010.5.peg.3445.
PATRIC18951904. VBIBacLic203714_3152.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
GeneTreeEBGT00050000002857.
HOGENOMHBG335267.
OMALATYDTG.
PhylomeDBQ65G67.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycBLIC279010-1:BLI03084-MONOMER.
BLIC279010:BL00417-MONOMER.

Family and domain databases

HAMAPMF_00005. Arg_succ_synth_type1.
[Tree]
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.90.1260.10. G3DSA:3.90.1260.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01940.
PANTHERPTHR11587. Arginosuc_synth. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. ArgG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_BACLD
AccessionPrimary (citable) accession number: Q65G67
Secondary accession number(s): Q62RM1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 25, 2004
Last modified: January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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