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Protein

NAD kinase 2

Gene

nadK2

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511Proton acceptorUniRule annotation
Binding sitei149 – 1491NADUniRule annotation
Binding sitei151 – 1511NADUniRule annotation
Binding sitei186 – 1861NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 522NADUniRule annotation
Nucleotide bindingi122 – 1232NADUniRule annotation
Nucleotide bindingi162 – 1676NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-3067-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 2UniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinase 2UniRule annotation
Gene namesi
Name:nadK2UniRule annotation
Ordered Locus Names:BLi03093, BL00367
OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265NAD kinase 2PRO_0000229605Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279010.BL00367.

Structurei

3D structure databases

ProteinModelPortaliQ65G58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiNTIMHEN.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65G58-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRRNIYFF YKKSEETDEQ CKKLKQLAEE HGFRVVHHHN EANIIASIGG
60 70 80 90 100
DGTFLQAVRK TNFRDDCLYV GVAKKGKAHL YADFNIYDTD KMIEATNSEQ
110 120 130 140 150
IEVRKYPLID VTVDGTSFQC LNEVSIRSSI IKTFVMDVYI DDLHFETFRG
160 170 180 190 200
DGMIVSTPTG STAYNKSVDG AIVDPLIPCI QVTELASLNN NTYRTLGSPF
210 220 230 240 250
ILSADRKLTL KIVQDGNDYP IIGLDNEAFS TMNVKEVEVS LSGKMIKTIK
260
LKDNSFWEKV RRTFL
Length:265
Mass (Da):30,063
Last modified:October 25, 2004 - v1
Checksum:i664FE2095DC6A18F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU41956.1.
CP000002 Genomic DNA. Translation: AAU24598.1.
RefSeqiYP_006714427.1. NC_006322.1.
YP_080236.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU24598; AAU24598; BL00367.
AAU41956; AAU41956; BLi03093.
GeneIDi3028995.
3098741.
KEGGibld:BLi03093.
bli:BL00367.
PATRICi18951924. VBIBacLic203714_3162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU41956.1.
CP000002 Genomic DNA. Translation: AAU24598.1.
RefSeqiYP_006714427.1. NC_006322.1.
YP_080236.1. NC_006270.3.

3D structure databases

ProteinModelPortaliQ65G58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BL00367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU24598; AAU24598; BL00367.
AAU41956; AAU41956; BLi03093.
GeneIDi3028995.
3098741.
KEGGibld:BLi03093.
bli:BL00367.
PATRICi18951924. VBIBacLic203714_3162.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiNTIMHEN.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-3067-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 13 / ATCC 14580.

Entry informationi

Entry nameiNADK2_BACLD
AccessioniPrimary (citable) accession number: Q65G58
Secondary accession number(s): Q62RL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 25, 2004
Last modified: January 7, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.