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Q65G12

- TRMB_BACLD

UniProt

Q65G12 - TRMB_BACLD

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bacillus licheniformis (strain DSM 13 / ATCC 14580)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (25 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441S-adenosyl-L-methionineUniRule annotation
    Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
    Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
    Active sitei118 – 1181By similarity
    Binding sitei118 – 1181S-adenosyl-L-methionineUniRule annotation
    Binding sitei122 – 1221SubstrateUniRule annotation
    Binding sitei154 – 1541SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBLIC279010:GJ2P-3113-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:BLi03141, BL00042
    OrganismiBacillus licheniformis (strain DSM 13 / ATCC 14580)
    Taxonomic identifieri279010 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000000606: Chromosome, UP000000608: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213tRNA (guanine-N(7)-)-methyltransferasePRO_0000229154Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi279010.BL00042.

    Structurei

    3D structure databases

    ProteinModelPortaliQ65G12.
    SMRiQ65G12. Positions 10-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1296Interaction with RNAUniRule annotation
    Regioni191 – 1944Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiDNVMTEY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q65G12-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRMRHKPWAD DYLAENSHIV ISEPSQYKGK WHSVFGNDNP IHIEVGTGKG    50
    QFISGMALQN PDVNYIGIEL FKSVIVTAVD KVKQTEAPNV KLLNINANML 100
    SDVFADGEVD RVYLNFSDPW PKKRHEKRRL TNHAFLKKYE QVLGGKGAIH 150
    FKTDNRGLFE YSLTSFSEYG LVLTFVSLDL HQSDFEGNVM TEYEEKFAAK 200
    GQPIYRVEAE WRT 213
    Length:213
    Mass (Da):24,431
    Last modified:October 25, 2004 - v1
    Checksum:iDABC74446D43BA28
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017333 Genomic DNA. Translation: AAU42002.1.
    CP000002 Genomic DNA. Translation: AAU24643.1.
    RefSeqiYP_006714473.1. NC_006322.1.
    YP_080281.1. NC_006270.3.

    Genome annotation databases

    EnsemblBacteriaiAAU24643; AAU24643; BL00042.
    AAU42002; AAU42002; BLi03141.
    GeneIDi3027940.
    3098881.
    KEGGibld:BLi03141.
    bli:BL00042.
    PATRICi18952020. VBIBacLic203714_3210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017333 Genomic DNA. Translation: AAU42002.1 .
    CP000002 Genomic DNA. Translation: AAU24643.1 .
    RefSeqi YP_006714473.1. NC_006322.1.
    YP_080281.1. NC_006270.3.

    3D structure databases

    ProteinModelPortali Q65G12.
    SMRi Q65G12. Positions 10-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 279010.BL00042.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU24643 ; AAU24643 ; BL00042 .
    AAU42002 ; AAU42002 ; BLi03141 .
    GeneIDi 3027940.
    3098881.
    KEGGi bld:BLi03141.
    bli:BL00042.
    PATRICi 18952020. VBIBacLic203714_3210.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi DNVMTEY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci BLIC279010:GJ2P-3113-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
      Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 13 / ATCC 14580.

    Entry informationi

    Entry nameiTRMB_BACLD
    AccessioniPrimary (citable) accession number: Q65G12
    Secondary accession number(s): Q62RG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: October 25, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3