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Q65FX8 (SYL_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BLi03175, BL00016
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151971

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65FX8 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 8771DEBA2523A0EB

FASTA80491,635
        10         20         30         40         50         60 
MSFDHQTIEK KWQDYWLKNK TFATREDKSK PKFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAVFT EQNIDNFRRQ IRALGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPD YYKWTQWIFL KLYEKGLAYV DEVPVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVERRPMKQW MLKITAYADR LLEDLEELDW PESIKEMQRN WIGRSEGAHV HFAVDGTNET 

       250        260        270        280        290        300 
FTVFTTRPDT LFGATYAVLA PEHDLVQKIT TPEQKDAVNA YIEEVQSKSD LERTDLAKTK 

       310        320        330        340        350        360 
TGVFTGAYAV NPANGEKIPV WIADYVLATY GTGAVMAVPA HDERDHEFAS AFGLPIKEVV 

       370        380        390        400        410        420 
KGGDVEKEAY TGDGEHIHSD FLNGLDKAAA IEKMIQWLEE NGKGEKKVTY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGTSTPV PEEELPLILP KTSEIKPSGT GESPLANIKE WVEVTDPETG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSCWYF LRYIDPKNPN ELASPEKLKE WLPVDMYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLYD IGVVPTKEPF KQLYNQGMIL GENNEKMSKS KGNVVNPDEI VETHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASIAWSTTGL DGARRFLDRV WRLFIDDNGG LNEKIVEGAG ETLERVYHET 

       670        680        690        700        710        720 
VMKVTDHFEG LRFNTGISQL MVFINEAYKA DQLPKEYMEG FVKLLSPVAP HLAEELWNRL 

       730        740        750        760        770        780 
GHEETIAYEA WPVYDEAKLV DDEIEIVVQL NGKVKAKLNV PADADKEQLE ELAKNNEKVK 

       790        800 
EQLEGKTIRK VIAVPGKLVN IVAN 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017333 Genomic DNA. Translation: AAU42036.1.
CP000002 Genomic DNA. Translation: AAU24674.1.
RefSeqYP_006714507.1. NC_006322.1.
YP_080312.1. NC_006270.3.

3D structure databases

ProteinModelPortalQ65FX8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL00016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU24674; AAU24674; BL00016.
AAU42036; AAU42036; BLi03175.
GeneID3028705.
3098985.
KEGGbld:BLi03175.
bli:BL00016.
PATRIC18952092. VBIBacLic203714_3246.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-3147-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BACLD
AccessionPrimary (citable) accession number: Q65FX8
Secondary accession number(s): Q62RD6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 25, 2004
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries