Q65FV7 (PCKA_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase

Gene names

Name:	pckA
Ordered Locus Names:	BLi03197, BL00839

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus licheniformis

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453		PRO_0000236919
Regions
	Nucleotide binding	233 – 240	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65FV7 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: BE2002BCB9FF58C0	FASTA	527	58,167
	10 20 30 40 50 60 MNSVDLTTDL QTLLTGENVQ MNLSVPHLVE KILERKEGVL TSSGAVRATT GTYTGRSPKD 70 80 90 100 110 120 KFIVEEASTK DKIDWGAVNQ PISEAAFDRL YAKVLGYLKE RDELYVFEGF AGADEKYRLP 130 140 150 160 170 180 ITVVNEFAWH NLFARQLFIR PEEHDKKPEE QPFTILSAPH FKADPLVDGT RSETFIIVSF 190 200 210 220 230 240 EKRTILIGGT EYAGEMKKSI FSIMNFLLPE QNILPMHCSA NIGEEGGTAL FFGLSGTGKT 250 260 270 280 290 300 TLSADPKRKL IGDDEHGWSS TGVFNIEGGC YAKCINLSEE KEPQIYNAIR FGSVLENVVV 310 320 330 340 350 360 DSKTGEPDYS DSFYTENTRA AYPIDAIDNI VKPSIAAHPQ TIVFLTADAF GVLPPISKLT 370 380 390 400 410 420 KEQAMYHFLS GYTSKLAGTE RGITTPEATF STCFGSPFLP LPAHVYAEML GRKIDEHGVG 430 440 450 460 470 480 VFLVNTGWTG GGYGTGERMS LAYTRAMVQA AIEGELDKAE MRTDRIFGLH SPVHVPGVPD 490 500 510 520 QVLDPAKTWT DENEYEEKAI HLAKAFKQNF KKFSNTENIE KAGGPLV

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU42057.1. CP000002 Genomic DNA. Translation: AAU24695.1.
RefSeq	YP_006714528.1. NC_006322.1. YP_080333.1. NC_006270.3.
3D structure databases
ProteinModelPortal	Q65FV7.
SMR	Q65FV7. Positions 16-523.
ModBase	Search...
Protein-protein interaction databases
STRING	279010.BL00839.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU24695; AAU24695; BL00839. AAU42057; AAU42057; BLi03197.
GeneID	3027890. 3099051.
KEGG	bld:BLi03197. bli:BL00839.
PATRIC	18952140. VBIBacLic203714_3270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HOG000271471.
KO	K01610.
OMA	HCWSENG.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	BLIC279010:GJ2P-3168-MONOMER.
UniPathway	UPA00138.
Family and domain databases
Gene3D	3.40.449.10. 1 hit. 3.90.228.20. 2 hits.
HAMAP	MF_00453. PEPCK_ATP.
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. [Graphical view]
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. pckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA_BACLD

Accession

Primary (citable) accession number: Q65FV7
Secondary accession number(s): Q62RB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program