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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation
Metal bindingi198 – 1981ManganeseUniRule annotation
Binding sitei198 – 1981ATPUniRule annotation
Binding sitei198 – 1981SubstrateUniRule annotation
Metal bindingi217 – 2171Manganese; via tele nitrogenUniRule annotation
Binding sitei217 – 2171ATPUniRule annotation
Metal bindingi254 – 2541ManganeseUniRule annotation
Binding sitei282 – 2821ATPUniRule annotation
Binding sitei319 – 3191ATPUniRule annotation
Binding sitei319 – 3191SubstrateUniRule annotation
Binding sitei444 – 4441ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi233 – 2419ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-3168-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:BLi03197, BL00839
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Phosphoenolpyruvate carboxykinase [ATP]PRO_0000236919Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279010.BLi03197.

Structurei

3D structure databases

ProteinModelPortaliQ65FV7.
SMRiQ65FV7. Positions 16-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1866.
HOGENOMiHOG000271471.
KOiK01610.
OMAiVRVITEV.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q65FV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSVDLTTDL QTLLTGENVQ MNLSVPHLVE KILERKEGVL TSSGAVRATT
60 70 80 90 100
GTYTGRSPKD KFIVEEASTK DKIDWGAVNQ PISEAAFDRL YAKVLGYLKE
110 120 130 140 150
RDELYVFEGF AGADEKYRLP ITVVNEFAWH NLFARQLFIR PEEHDKKPEE
160 170 180 190 200
QPFTILSAPH FKADPLVDGT RSETFIIVSF EKRTILIGGT EYAGEMKKSI
210 220 230 240 250
FSIMNFLLPE QNILPMHCSA NIGEEGGTAL FFGLSGTGKT TLSADPKRKL
260 270 280 290 300
IGDDEHGWSS TGVFNIEGGC YAKCINLSEE KEPQIYNAIR FGSVLENVVV
310 320 330 340 350
DSKTGEPDYS DSFYTENTRA AYPIDAIDNI VKPSIAAHPQ TIVFLTADAF
360 370 380 390 400
GVLPPISKLT KEQAMYHFLS GYTSKLAGTE RGITTPEATF STCFGSPFLP
410 420 430 440 450
LPAHVYAEML GRKIDEHGVG VFLVNTGWTG GGYGTGERMS LAYTRAMVQA
460 470 480 490 500
AIEGELDKAE MRTDRIFGLH SPVHVPGVPD QVLDPAKTWT DENEYEEKAI
510 520
HLAKAFKQNF KKFSNTENIE KAGGPLV
Length:527
Mass (Da):58,167
Last modified:October 25, 2004 - v1
Checksum:iBE2002BCB9FF58C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU42057.1.
CP000002 Genomic DNA. Translation: AAU24695.1.
RefSeqiWP_003184588.1. NC_006322.1.
YP_006714528.1. NC_006322.1.
YP_080333.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU24695; AAU24695; BL00839.
AAU42057; AAU42057; BLi03197.
GeneIDi3027890.
KEGGibld:BLi03197.
bli:BL00839.
PATRICi18952140. VBIBacLic203714_3270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA. Translation: AAU42057.1.
CP000002 Genomic DNA. Translation: AAU24695.1.
RefSeqiWP_003184588.1. NC_006322.1.
YP_006714528.1. NC_006322.1.
YP_080333.1. NC_006270.3.

3D structure databases

ProteinModelPortaliQ65FV7.
SMRiQ65FV7. Positions 16-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi03197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU24695; AAU24695; BL00839.
AAU42057; AAU42057; BLi03197.
GeneIDi3027890.
KEGGibld:BLi03197.
bli:BL00839.
PATRICi18952140. VBIBacLic203714_3270.

Phylogenomic databases

eggNOGiCOG1866.
HOGENOMiHOG000271471.
KOiK01610.
OMAiVRVITEV.
OrthoDBiEOG6DG2RK.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciBLIC279010:GJ2P-3168-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46.

Entry informationi

Entry nameiPCKA_BACLD
AccessioniPrimary (citable) accession number: Q65FV7
Secondary accession number(s): Q62RB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 25, 2004
Last modified: June 24, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.