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Q65FS4 (GLGB_BACLD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:BLi03230, BL01419
OrganismBacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]
Taxonomic identifier279010 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length627 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6276271,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188681

Sites

Active site3091Nucleophile By similarity
Active site3521Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q65FS4 [UniParc].

Last modified October 25, 2004. Version 1.
Checksum: 15A2BBE6AFD55161

FASTA62773,820
        10         20         30         40         50         60 
MAGVSASDIK AHLFHEGRLY ESYRFFGAHQ TEQDGRQGFR FCVWAPHAKE VRIAGTFNGW 

        70         80         90        100        110        120 
SGVLHRMEKR HQEGIWELFI PGIGEGELYK YEMITAANET KLKADPYAFF SEVRPKTASV 

       130        140        150        160        170        180 
TYQLAGYQWG DRKWQKKKQQ KAVYEKPISI YELHIGSWKK KADGRFFTYR ELSETVIPYV 

       190        200        210        220        230        240 
KEHGFTHIEL MPLTEHPFDR SWGYQTTGYY SPTSRYGEPH DLMYFIDQCH QHEIGVIMDW 

       250        260        270        280        290        300 
VSGHFCKDDH GLYLFDGMPL YEYKHEHDRE NWEWGTANFD LGKREVHSFL ISNALYWIEM 

       310        320        330        340        350        360 
YHIDGFRVDA VANLLYWPNR PQPEANGFAI EFIQTLNEQV FAKDPHFLMI AEDSTDWPLV 

       370        380        390        400        410        420 
THPTYEGGLG FNYKWNMGWM NDVLTYMEAS PEQRKHLHHL VSFSLMYAYS ENYILPFSHD 

       430        440        450        460        470        480 
EVVYRKKSLL HKMPGDYWQK FAQYRLLISY FMMHPGKKLL FMGGEFAQFD EWKDEEELDW 

       490        500        510        520        530        540 
FLDDFDMHRK ARMFTKEALH LYKKSRILYE NDHRQQGFEW IDVNNAEQSI VSFIRYGKQP 

       550        560        570        580        590        600 
GEALIIVCNF TPSVYHEYRV GVPFQTEYIE VLNSDDAKYG GSHQINPKRL QARKGVLHGK 

       610        620 
PYSISMTVPP LGAAVFRAVK KRGAKKG 

« Hide

References

[1]"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.
[2]"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species."
Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. expand/collapse author list , Galleron N., Ehrlich S.D., Berka R.M.
Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017333 Genomic DNA. Translation: AAU42090.1.
CP000002 Genomic DNA. Translation: AAU24729.1.
RefSeqYP_006714563.1. NC_006322.1.
YP_080367.1. NC_006270.3.

3D structure databases

ProteinModelPortalQ65FS4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279010.BL01419.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEQ65FS4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU24729; AAU24729; BL01419.
AAU42090; AAU42090; BLi03230.
GeneID3027988.
3099151.
KEGGbld:BLi03230.
bli:BL01419.
PATRIC18952212. VBIBacLic203714_3306.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBLIC279010:GJ2P-3202-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BACLD
AccessionPrimary (citable) accession number: Q65FS4
Secondary accession number(s): Q62R81
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 25, 2004
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries