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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei309NucleophileUniRule annotation1
Active sitei352Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBLIC279010:G1G12-3264-MONOMER
UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:BLi03230, BL01419
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000000606 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001886811 – 6271,4-alpha-glucan branching enzyme GlgBAdd BLAST627

Proteomic databases

PRIDEiQ65FS4

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi03230

Structurei

3D structure databases

ProteinModelPortaliQ65FS4
SMRiQ65FS4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C Bacteria
COG0296 LUCA
HOGENOMiHOG000283037
KOiK00700
OMAiEVVHGKS

Family and domain databases

Gene3Di2.60.40.101 hit
2.60.40.11801 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

Q65FS4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVSASDIK AHLFHEGRLY ESYRFFGAHQ TEQDGRQGFR FCVWAPHAKE
60 70 80 90 100
VRIAGTFNGW SGVLHRMEKR HQEGIWELFI PGIGEGELYK YEMITAANET
110 120 130 140 150
KLKADPYAFF SEVRPKTASV TYQLAGYQWG DRKWQKKKQQ KAVYEKPISI
160 170 180 190 200
YELHIGSWKK KADGRFFTYR ELSETVIPYV KEHGFTHIEL MPLTEHPFDR
210 220 230 240 250
SWGYQTTGYY SPTSRYGEPH DLMYFIDQCH QHEIGVIMDW VSGHFCKDDH
260 270 280 290 300
GLYLFDGMPL YEYKHEHDRE NWEWGTANFD LGKREVHSFL ISNALYWIEM
310 320 330 340 350
YHIDGFRVDA VANLLYWPNR PQPEANGFAI EFIQTLNEQV FAKDPHFLMI
360 370 380 390 400
AEDSTDWPLV THPTYEGGLG FNYKWNMGWM NDVLTYMEAS PEQRKHLHHL
410 420 430 440 450
VSFSLMYAYS ENYILPFSHD EVVYRKKSLL HKMPGDYWQK FAQYRLLISY
460 470 480 490 500
FMMHPGKKLL FMGGEFAQFD EWKDEEELDW FLDDFDMHRK ARMFTKEALH
510 520 530 540 550
LYKKSRILYE NDHRQQGFEW IDVNNAEQSI VSFIRYGKQP GEALIIVCNF
560 570 580 590 600
TPSVYHEYRV GVPFQTEYIE VLNSDDAKYG GSHQINPKRL QARKGVLHGK
610 620
PYSISMTVPP LGAAVFRAVK KRGAKKG
Length:627
Mass (Da):73,820
Last modified:October 25, 2004 - v1
Checksum:i15A2BBE6AFD55161
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017333 Genomic DNA Translation: AAU42090.1
CP000002 Genomic DNA Translation: AAU24729.1
RefSeqiWP_009329438.1, NC_006322.1

Genome annotation databases

EnsemblBacteriaiAAU24729; AAU24729; BL01419
AAU42090; AAU42090; BLi03230
GeneIDi3027988
KEGGibld:BLi03230
bli:BL01419
PATRICifig|279010.13.peg.3303

Similar proteinsi

Entry informationi

Entry nameiGLGB_BACLD
AccessioniPrimary (citable) accession number: Q65FS4
Secondary accession number(s): Q62R81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 25, 2004
Last modified: February 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome