Q65FC4 (KHSE_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	BLi03412, BL02135

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Homoserine kinase HAMAP MF_00384		PRO_1000049109
Regions
	Nucleotide binding	91 – 101	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65FC4 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 71C739B8211250FD	FASTA	306	33,064
	10 20 30 40 50 60 MTEADMLFSV TVPGSTANLG PGFDSVGMAL SRYLRLSVFP HDEWRFEAET EVVAGIPEGT 70 80 90 100 110 120 DNLIYQVAKR TAAHFGKELP PSLVKVWSDI PLARGLGSSA AAIAAAVELA NELADLKLSD 130 140 150 160 170 180 REKLHFASLE EGHPDNAGAS LFGGLVIGLH EEDETEMVSM KDIDLDVVVV IPFYEVLTKD 190 200 210 220 230 240 ARDVLPESLS YPKAVEASAV SNMLVAGLMA KDWKLVGRMM QKDLFHQPYR RALVPELSKV 250 260 270 280 290 300 EHEAGQNGAF GTALSGAGPT ILSFIEKGKG EALRNQLASK FPHCEVDCLY VPDTGIIVER KSVNSV

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000002 Genomic DNA. Translation: AAU24871.1. AE017333 Genomic DNA. Translation: AAU42240.1.
RefSeq	YP_080509.1. NC_006270.3. YP_092933.1. NC_006322.1.
3D structure databases
HSSP	HSSP built from PDB template 1H74 based on UniProtKB Q58504.
ProteinModelPortal	Q65FC4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65FC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000055356; EBBACP00000053887; EBBACG00000055347. EBBACT00000060707; EBBACP00000059140; EBBACG00000060698.
GeneID	3027602. 3099765.
GenomeReviews	Gene locus BLi03412 in contig AE017333_GR. Gene locus BL02135 in contig CP000002_GR.
KEGG	bld:BLi03412. bli:BL02135.
NMPDR	fig|279010.5.peg.3658.
PATRIC	18952572. VBIBacLic203714_3462.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000000874.
HOGENOM	HBG646290.
OMA	DRLHQPY.
PhylomeDB	Q65FC4.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI03412-MONOMER. BLIC279010:BL02135-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_BACLD

Accession

Primary (citable) accession number: Q65FC4
Secondary accession number(s): Q62QT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program