Reviewed, UniProtKB/Swiss-Prot Q65EN0 (TPIS_BACLD)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	BLi03663, BL03466

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Triosephosphate isomerase HAMAP MF_00147		PRO_0000307428
Sites
	Active site	95	1	Electrophile By similarity
	Active site	167	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity
Amino acid modifications
	Modified residue	213	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65EN0-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 4817643B31F6CC48	FASTA	253	27,014
	10 20 30 40 50 60 MRKPIIAGNW KMNKVLSEAV SFVEEVKSSI PAADKAEAVV CAPALFLEKL TSAVKGTDLK 70 80 90 100 110 120 VGAQNMHFEE SGAFTGEISP AALKDLGVEY CVIGHSERRE MFAETDETVN KKAHAAFKYG 130 140 150 160 170 180 IVPIICVGET LEEREANKTN ELVADQVKKA LAGLTTEQVA ASVIAYEPIW AIGTGKSSTA 190 200 210 220 230 240 QDANEVCAHI RKTVASEFGQ TAADSVRIQY GGSVKPANIK EYMAESDIDG ALVGGASLEP 250 QSFVQLLEAG QYE

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:RESEARCH077.1-RESEARCH077.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000002 Genomic DNA. Translation: AAU25113.1. AE017333 Genomic DNA. Translation: AAU42484.1.
RefSeq	YP_080751.1. YP_093177.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q65EN0.
Genome annotation databases
GeneID	3029331. 3100631.
GenomeReviews	Gene locus BLi03663 in contig AE017333_GR. Gene locus BL03466 in contig CP000002_GR.
KEGG	bld:BLi03663. bli:BL03466.
NMPDR	fig|279010.5.peg.2314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q65EN0.
OMA	NELVADQ.
Enzyme and pathway databases
BioCyc	BLIC279010:BL03466-MON.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BACLD

Accession

Primary (citable) accession number: Q65EN0
Secondary accession number(s): Q62Q47

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 25, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)