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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathway: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateUniRule annotation
Binding sitei118 – 1181SubstrateUniRule annotation
Binding sitei151 – 1511SubstrateUniRule annotation
Binding sitei201 – 2011ATPUniRule annotation
Binding sitei323 – 3231ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi350 – 3534ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBLIC279010:GJ2P-3623-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinaseUniRule annotation (EC:2.7.2.3UniRule annotation)
Gene namesi
Name:pgkUniRule annotation
Ordered Locus Names:BLi03664, BL03465
OrganismiBacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46)
Taxonomic identifieri279010 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000000606 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Phosphoglycerate kinasePRO_1000057966Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphoserineUniRule annotation
Modified residuei299 – 2991PhosphothreonineUniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ65EM9.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi279010.BLi03664.

Structurei

3D structure databases

ProteinModelPortaliQ65EM9.
SMRiQ65EM9. Positions 1-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 233Substrate bindingUniRule annotation
Regioni59 – 624Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227108.
KOiK00927.
OMAiWEALDIG.
OrthoDBiEOG64N9Z0.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q65EM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKSVKDID VKGRVVFCRV DFNVPMKDGK VTDDTRIRAA LPTIQYLTEQ
60 70 80 90 100
GAKVLLASHL GRPKGQVSEE LRLTPVAARL GELIGKEVKK ADEAYGDAVK
110 120 130 140 150
AQISEMKDGD ILVLENVRFY PGEEKNDPEL SKAFADLADV YVNDAFGAAH
160 170 180 190 200
RAHASTAGIA EHLPAVAGFL MEKELEVLGK ALSNPERPFT AIIGGAKVKD
210 220 230 240 250
KIGVIESLLE KVDNLIIGGG LAYTFVKALG HEVGKSLLEE DKIELAKSFM
260 270 280 290 300
DRAKEKGVNF YMPTDVLVAD DFSNDANTKI VPVSEIPSDW EALDIGEETR
310 320 330 340 350
KTYADVIKNS KLVVWNGPMG VFEIDAFAKG TRAVAEALAE ANDTYSVIGG
360 370 380 390
GDSAAAVEKF GLADKMSHIS TGGGASLEFM EGKELPGVKA LNDK
Length:394
Mass (Da):42,382
Last modified:October 25, 2004 - v1
Checksum:i8E7C195741A960EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU25114.1.
AE017333 Genomic DNA. Translation: AAU42485.1.
RefSeqiWP_003185465.1. NC_006322.1.
YP_006714948.1. NC_006322.1.
YP_080752.1. NC_006270.3.

Genome annotation databases

EnsemblBacteriaiAAU25114; AAU25114; BL03465.
AAU42485; AAU42485; BLi03664.
GeneIDi3029334.
KEGGibld:BLi03664.
bli:BL03465.
PATRICi18953114. VBIBacLic203714_3733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000002 Genomic DNA. Translation: AAU25114.1.
AE017333 Genomic DNA. Translation: AAU42485.1.
RefSeqiWP_003185465.1. NC_006322.1.
YP_006714948.1. NC_006322.1.
YP_080752.1. NC_006270.3.

3D structure databases

ProteinModelPortaliQ65EM9.
SMRiQ65EM9. Positions 1-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279010.BLi03664.

Proteomic databases

PRIDEiQ65EM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU25114; AAU25114; BL03465.
AAU42485; AAU42485; BLi03664.
GeneIDi3029334.
KEGGibld:BLi03664.
bli:BL03465.
PATRICi18953114. VBIBacLic203714_3733.

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227108.
KOiK00927.
OMAiWEALDIG.
OrthoDBiEOG64N9Z0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciBLIC279010:GJ2P-3623-MONOMER.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential."
    Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46.

Entry informationi

Entry nameiPGK_BACLD
AccessioniPrimary (citable) accession number: Q65EM9
Secondary accession number(s): Q62Q46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 25, 2004
Last modified: June 24, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.