Reviewed, UniProtKB/Swiss-Prot Q65EF5 (PELC_BACLD)

Names and origin

Protein names

Recommended name:
    Pectate lyase C
    EC=4.2.2.2
Alternative name(s):
    Pectin lyase
    EC=4.2.2.10

Gene names

Name:	pelC
Synonyms:	yvpA
Ordered Locus Names:	BLi03741, BL03597

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins By similarity.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends. Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the polysaccharide lyase 3 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC pectin lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 221	193	Pectate lyase C		PRO_0000233103

Sequences

	Sequence		Length	Mass (Da)
	Q65EF5-1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: B15454293DC27FC4	FASTA	221	23,618
	10 20 30 40 50 60 MKRLAGTVIL SGLLVCGFGQ ALPEKALAAE VVHKTIVVEK GQTYDGKGKR LIAGPELGDG 70 80 90 100 110 120 SQREDQKPIF KVEDGATLKN VVLGAPAADG VHTYGNASIN NVVWEDVGED ALTVKSEGSV 130 140 150 160 170 180 TINGGSARLA ADKIFQINKA STFTVKNFTA DQGGKFIRQL GGSTFKAVVN IDNCTITNMK 190 200 210 220 EAIFRTDSST SSVTMTNTRY SKVGQKWIGV KHATERNNHE F

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU42559.1. Different initiation. CP000002 Genomic DNA. Translation: AAU25188.1.
RefSeq	YP_080826.1. YP_093252.1.
3D structure databases
SMR	Q65EF5. Positions 28-211.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65EF5.
Protein family/group databases
CAZy	PL3. Polysaccharide Lyase Family 3.
Genome annotation databases
GeneID	3028303. 3100937.
GenomeReviews	Gene locus BLi03741 in contig AE017333_GR. Gene locus BL03597 in contig CP000002_GR.
KEGG	bld:BLi03741. bli:BL03597.
NMPDR	fig|279010.5.peg.3805.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG05501.
HOGENOM	HBG349026.
OMA	EAIFRTD.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI03741-MONOMER. BLIC279010:BL03597-MONOMER.
Family and domain databases
InterPro	IPR004898. Pectate_lyase_cat. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF03211. Pectate_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PELC_BACLD

Accession

Primary (citable) accession number: Q65EF5
Secondary accession number(s): Q62PX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	January 19, 2010
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)