Q65E27 (MSCL_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	BLi03872, BL02495

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 126	126	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_0000237973
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	40 – 60	21	Helical; Potential
	Transmembrane	67 – 87	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q65E27 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 42F642822C40D042	FASTA	126	14,210
	10 20 30 40 50 60 MWKEFKSFAI RGNVIDLAIG VIIGGAFGKI VTSLVNDLMM PLLGLLLGGL DFSALSFTFV 70 80 90 100 110 120 DAEIKYGLFI QSIVNFFIIS FSIFLFIRYI SKLKKKDAEE EKAAPDPQEE LLKEIRDLLK EQTNRS

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed: 15383718] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed: 15461803] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU42687.1. CP000002 Genomic DNA. Translation: AAU25312.1.
RefSeq	YP_080950.1. NC_006270.3. YP_093380.1. NC_006322.1.
3D structure databases
ProteinModelPortal	Q65E27.
ModBase	Search...
Protein-protein interaction databases
STRING	Q65E27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000056733; EBBACP00000055264; EBBACG00000056724. EBBACT00000061885; EBBACP00000060318; EBBACG00000061876.
GeneID	3028707. 3101083.
GenomeReviews	Gene locus BLi03872 in contig AE017333_GR. Gene locus BL02495 in contig CP000002_GR.
KEGG	bld:BLi03872. bli:BL02495.
NMPDR	fig|279010.5.peg.4064.
PATRIC	18953538. VBIBacLic203714_3944.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1970.
GeneTree	EBGT00050000002096.
HOGENOM	HBG727604.
OMA	VIDFVIV.
ProtClustDB	PRK13955.
Enzyme and pathway databases
BioCyc	BLIC279010-1:BLI03872-MONOMER. BLIC279010:BL02495-MONOMER.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_BACLD

Accession

Primary (citable) accession number: Q65E27
Secondary accession number(s): Q62PJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program