Q65DU6 (KITH_BACLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	BLi03954, BL03975

Organism

Bacillus licheniformis (strain DSM 13 / ATCC 14580) [Complete proteome] [HAMAP]

Taxonomic identifier

279010 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus licheniformis

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000174961
Regions
	Nucleotide binding	15 – 22	8	ATP By similarity
	Nucleotide binding	88 – 91	4	ATP By similarity
Sites
	Active site	89	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	148	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q65DU6 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: CF99EAE939FD14A1	FASTA	194	21,298
	10 20 30 40 50 60 MYIMKQSGWL ELICGSMFSG KSEELIRRIK RATFAKQEVK VFKPAIDNRY SSESVVSHNG 70 80 90 100 110 120 TSIVCHAIAS PEEIFQYISK ETDVIGVDEV QFFDETIVGT LTSLADQGYR VIAAGLDLDF 130 140 150 160 170 180 RGEPFGVVPD LMALAETVTK LQAVCSVCGS PASRTQRLIN GKPASYDDPV ILVGASEAYE 190 ARCRHHHEVP GNPK

References

[1]

"The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential." Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P., Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G. J. Mol. Microbiol. Biotechnol. 7:204-211(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

[2]

"Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species." Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A. , Galleron N., Ehrlich S.D., Berka R.M. Genome Biol. 5:R77.1-R77.12(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 13 / ATCC 14580.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017333 Genomic DNA. Translation: AAU42768.1. CP000002 Genomic DNA. Translation: AAU25393.1.
RefSeq	YP_006715231.1. NC_006322.1. YP_081031.1. NC_006270.3.
3D structure databases
ProteinModelPortal	Q65DU6.
SMR	Q65DU6. Positions 1-191.
ModBase	Search...
Protein-protein interaction databases
STRING	279010.BL03975.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU25393; AAU25393; BL03975. AAU42768; AAU42768; BLi03954.
GeneID	3027778. 3101344.
KEGG	bld:BLi03954. bli:BL03975.
PATRIC	18953702. VBIBacLic203714_4026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076390.
KO	K00857.
OMA	ELICGSM.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	BLIC279010:GJ2P-3907-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_BACLD

Accession

Primary (citable) accession number: Q65DU6
Secondary accession number(s): Q62PB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 25, 2004
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program